Medical Biochemistry Department, National Research Center, 33 El Behouth St., 12622 Dokki, Giza, Egypt.
Pre-Treatment and Finishing of Cellulosic Fabric Department, Textile Research Division, National Research Centre, Dokki, Giza, Egypt.
Biomed Pharmacother. 2017 Jul;91:1006-1016. doi: 10.1016/j.biopha.2017.05.043. Epub 2017 May 15.
Dextran-capped gold nanoparticles (Au-dextran NPs) were prepared exploiting the natural polysaccharide polymer as both reducing and stabilizing agent in the synthesis process, aiming at studying their antitumor effect on solid carcinoma and EAC-bearing mice. To this end, Au-dextran NPs were designed via simple eco-friendly chemical reaction and they were characterized revealing the monodispersed particles with narrow distributed size of around 49nm with high negative charge. In vivo experiments were performed on mice. Biochemical analysis of liver and kidney functions and oxidation stress ratio in addition to histopathological investigations of such tumor tissues were done demonstrating the potentiality of Au-dextran NPs as antitumor agent. The obtained results revealed that EAC and solid tumors caused significant increase in liver and kidney functions, liver oxidant parameters, alpha feto protein levels and diminished liver antioxidant accompanied by positive expression of tumor protein p53 of liver while the treatment with Au-dextran NPs for both types caused improvement in liver and kidney functions, increased liver antioxidant, increased the expression level of B-cell lymphoma 2 gene and subsequently suppressed the apoptotic pathway. As a result, the obtained data provides significant antitumor effects of the Au-dextran NPs in both Ehrlich ascites and solid tumor in mice models.
葡聚糖包覆的金纳米粒子（Au-dextran NPs）是利用天然多糖聚合物作为还原剂和稳定剂在合成过程中制备的，旨在研究其对固体癌和 EAC 荷瘤小鼠的抗肿瘤作用。为此，通过简单的环保化学反应设计了 Au-dextran NPs，并对其进行了表征，结果表明 Au-dextran NPs 具有单分散性，粒径分布窄，约为 49nm，带高负电荷。在小鼠体内进行了实验。对肝肾功能和氧化应激比进行了生化分析，并对这些肿瘤组织进行了组织病理学研究，结果表明 Au-dextran NPs 具有作为抗肿瘤剂的潜力。结果表明，EAC 和实体瘤导致肝肾功能、肝氧化剂参数、甲胎蛋白水平显著升高，肝抗氧化剂减少，同时肝肿瘤蛋白 p53 表达阳性，而 Au-dextran NPs 对两种类型的治疗均导致肝肾功能改善，肝抗氧化剂增加，B 细胞淋巴瘤 2 基因表达水平增加，随后抑制凋亡途径。因此，研究结果表明，Au-dextran NPs 对 EAC 腹水瘤和实体瘤在小鼠模型中均具有显著的抗肿瘤作用。
