Kiripolsky Jeremy, Shen Long, Liang Yichen, Li Alisa, Suresh Lakshmanan, Lian Yun, Li Quan-Zhen, Gaile Daniel P, Kramer Jill M
Department of Oral Biology, School of Dental Medicine, University of Buffalo, The State University of New York, Buffalo, NY 14214, USA.
Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen 361003, China; Autoimmune Division, Trinity Biotech, 60 Pineview Drive, Buffalo, NY 14228, USA.
Clin Immunol. 2017 Oct;183:225-232. doi: 10.1016/j.clim.2017.04.009. Epub 2017 May 17.
Animal models that recapitulate human disease are crucial for the study of Sjögren's Syndrome (SS). While several SS mouse models exist, there are few primary SS (pSS) models that mimic systemic disease manifestations seen in humans. Similar to pSS patients, NOD.B10Sn-H2/J (NOD.B10) mice develop exocrine gland disease and anti-nuclear autoantibodies. However, the disease kinetics and spectrum of extra-glandular disease remain poorly characterized in this model. Our objective was to characterize local and systemic SS manifestations in depth in NOD.B10 female mice at early and late disease time points. To this end, sera, exocrine tissue, lung, and kidney were analyzed. NOD.B10 mice have robust lymphocytic infiltration of salivary and lacrimal tissue. In addition, they exhibit significant renal and pulmonary inflammation. We identified numerous autoantibodies, including those directed against salivary proteins. In conclusion, the NOD.B10 model recapitulates both local and systemic pSS disease and represents an excellent model for translational studies.
能够重现人类疾病的动物模型对于干燥综合征（SS）的研究至关重要。虽然存在几种SS小鼠模型，但很少有原发性SS（pSS）模型能够模拟人类所见的全身性疾病表现。与pSS患者相似，NOD.B10Sn-H2/J（NOD.B10）小鼠会出现外分泌腺疾病和抗核自身抗体。然而，该模型中外分泌腺外疾病的疾病动力学和范围仍缺乏充分的特征描述。我们的目标是在疾病早期和晚期时间点深入表征NOD.B10雌性小鼠的局部和全身性SS表现。为此，我们分析了血清、外分泌组织、肺和肾。NOD.B10小鼠的唾液和泪腺组织有大量淋巴细胞浸润。此外，它们还表现出明显的肾脏和肺部炎症。我们鉴定出了多种自身抗体，包括针对唾液蛋白的自身抗体。总之，NOD.B10模型重现了局部和全身性pSS疾病，是转化研究的优秀模型。
