Zilles K, Schleicher A, Rath M, Bauer A
Anatomical Institute, University of Cologne, Federal Republic of Germany.
Histochemistry. 1988;90(2):129-37. doi: 10.1007/BF00500977.
Quantitative receptor autoradiography on sections of the human brain raises methodical problems of which some are relevant also for studies in animal tissue, but others are unique in studies of human brain tissue. Procedures for the following methodical aspects are discussed: image analysis for quantitation of the regional distribution of receptor densities, saturation analysis on autoradiographs, influence of age and post-mortem delay and quenching of beta-radiation in brain tissue. The solutions proposed to these problems make receptor autoradiography in the human brain to a reliable method for studies of chemical neuroanatomy.
对人脑切片进行定量受体放射自显影会引发一些方法学问题，其中一些问题在动物组织研究中也存在相关性，但另一些问题在人脑组织研究中是独特的。本文讨论了以下方法学方面的程序：用于定量受体密度区域分布的图像分析、放射自显影片上的饱和分析、年龄和死后延迟的影响以及脑组织中β射线的淬灭。针对这些问题提出的解决方案使对人脑进行受体放射自显影成为化学神经解剖学研究的可靠方法。
