miR-196a2、miR-146a 和 miR-499 多态性与韩国人群哮喘表型的关联。
Association of the miR-196a2, miR-146a, and miR-499 Polymorphisms with Asthma Phenotypes in a Korean Population.
机构信息
Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, South Korea.
Department of Biomedical Sciences, Ajou University School of Medicine, Suwon, South Korea.
出版信息
Mol Diagn Ther. 2017 Oct;21(5):547-554. doi: 10.1007/s40291-017-0280-1.
BACKGROUND
MicroRNAs (miRNAs) modulate expressions of inflammatory genes, thereby regulating inflammatory responses. Single nucleotide polymorphisms (SNPs) in miRNAs could affect their efficiency in binding to messenger RNAs (mRNAs).
OBJECTIVE
We investigated the associations of miRNA SNPs with asthma phenotypes. miR-196a2 (rs11614913 T>C), miR-146a (rs2910164 C>G), and miR-499 (rs3746444 A>G) were genotyped in 347 asthma patients and 172 normal healthy controls (NCs).
RESULTS
The CT/CC genotype of miR-196a2 rs11614913 was associated with eosinophilic asthma (p = 0.004) and a higher sputum eosinophil count compared with the TT genotype (p = 0.003). The CG/GG genotype of miR-146a rs2910164 tended to be associated with higher bronchial hyperresponsiveness to methacholine (PC) compared with the CC genotype. The AG/GG genotype of miR-499 rs3746444 was associated with higher predicted values of forced expiratory volume in 1 s (%FEV) compared with the AA genotype (p = 0.008).
CONCLUSIONS
Genetic polymorphisms in miR-196a2, miR-146a, and miR-499 could be potential biomarkers for asthma phenotypes and targets for asthma treatments in a Korean population.
背景
微小 RNA（miRNA）调节炎症基因的表达，从而调节炎症反应。miRNA 中的单核苷酸多态性（SNP）可能会影响它们与信使 RNA（mRNA）结合的效率。
目的
我们研究了 miRNA SNP 与哮喘表型的关联。在 347 例哮喘患者和 172 例正常健康对照者（NC）中，对 miR-196a2（rs11614913 T>C）、miR-146a（rs2910164 C>G）和 miR-499（rs3746444 A>G）进行了基因分型。
结果
miR-196a2 rs11614913 的 CT/CC 基因型与嗜酸性粒细胞性哮喘（p=0.004）和痰中嗜酸性粒细胞计数高于 TT 基因型（p=0.003）相关。miR-146a rs2910164 的 CG/GG 基因型与对乙酰甲胆碱的支气管高反应性（PC）升高有关，与 CC 基因型相比。miR-499 rs3746444 的 AG/GG 基因型与预测值的用力呼气量 1 秒（%FEV）高于 AA 基因型有关（p=0.008）。
结论
miR-196a2、miR-146a 和 miR-499 的遗传多态性可能是韩国人群哮喘表型的潜在生物标志物，也是哮喘治疗的靶点。
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