Serviço de Imunologia Basica e Clinica, Departamento de Patologia, Faculdade de Medicina da Universidade do Porto, 4200-319 Porto, Portugal.
EPIUnit-Instituto de Saude Publica, Universidade do Porto, 4200-450 Porto, Portugal.
Nutrients. 2022 Mar 8;14(6):1147. doi: 10.3390/nu14061147.
Individual nutrients and bioactive compounds have been implicated in the expression of microRNAs (miRNAs), which are related to inflammation and asthma. However, evidence about the impact of diet is scarce. Therefore, we aimed to assess the association between dietary acid load and asthma-related miRNA in the exhaled breath condensate (EBC) of school-aged children. This cross-sectional analysis included 150 participants aged 7 to 12 years (52% girls) from a nested case-control study, which randomly selected 186 children attending 71 classrooms from 20 public schools located in city of Porto, Portugal. Dietary data were collected by one 24 h-recall questionnaire. Dietary acid load was assessed using the potential renal acid load (PRAL) and net endogenous acid production (NEAP) scores. Based on previous studies, eleven asthma-related miRNAs were chosen and analyzed in EBC by reverse transcription-quantitative real-time PCR. PRAL, NEAP and miRNAs were categorized as high or low according to the median. Logistic regression models were performed to assess the association between dietary acid load scores and miRNAs. Children in high dietary acid load groups (PRAL ≥ 14.43 and NEAP ≥ 55.79 mEq/day) have significantly increased odds of having high miR-133a-3p levels. In conclusion, higher dietary acid loads possibly modulate asthma-related miRNAs of school-aged children.
个体营养素和生物活性化合物与 microRNAs（miRNAs）的表达有关，miRNAs 与炎症和哮喘有关。然而，关于饮食影响的证据很少。因此，我们旨在评估学龄儿童呼出气冷凝物（EBC）中饮食酸负荷与哮喘相关 miRNA 之间的关联。这项横断面分析包括来自嵌套病例对照研究的 150 名年龄在 7 至 12 岁的参与者（52%为女孩），该研究从葡萄牙波尔图市的 20 所公立学校的 71 个教室中随机选择了 186 名儿童。饮食数据通过一份 24 小时回顾性问卷收集。使用潜在肾酸负荷（PRAL）和净内源性酸产生（NEAP）评分评估饮食酸负荷。基于先前的研究，选择了 11 种与哮喘相关的 miRNA，并通过逆转录定量实时 PCR 分析 EBC 中的 miRNA。根据中位数将 PRAL、NEAP 和 miRNA 分为高或低。使用逻辑回归模型评估饮食酸负荷评分与 miRNA 之间的关联。饮食酸负荷较高组（PRAL≥14.43 和 NEAP≥55.79 mEq/天）的儿童，miR-133a-3p 水平较高的可能性显著增加。总之，较高的饮食酸负荷可能会调节学龄儿童与哮喘相关的 miRNA。
