Neshastehriz Ali, Tabei Mousa, Maleki Shayan, Eynali Samira, Shakeri-Zadeh Ali
Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran; Radiation Sciences Department, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Radiation Sciences Department, Iran University of Medical Sciences (IUMS), Tehran, Iran.
J Photochem Photobiol B. 2017 Jul;172:52-60. doi: 10.1016/j.jphotobiol.2017.05.012. Epub 2017 May 12.
The aim of this study was to develop an optimized method for preparation of folate conjugated gold nanoparticles (F-AuNPs) and to investigate its cytotoxic effects and cell apoptosis in combination with photothermal therapy (PTT) and radiotherapy (RT) for the treatment of mouth epidermal carcinoma cells KB. For this purpose, cells were treated with synthesized F-AuNPs at different concentrations for 6h and then irradiated them with laser beam (532nm, 0.5W/cm, 15min). After photothermal therapy, the cells were exposed to 6MV X-ray with a single dose of 2Gy. MTT assay were performed to evaluate the cell survival rate and apoptosis was determined by flow cytometry using an annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit. No significant cell damage or cell apoptosis from the individual treatment of laser light or F-AuNPs was observed, while viability of cells incubated with F-AuNPs and then exposed to the laser was significantly decreased. Additionally, our results demonstrated that F-AuNPs is good radiosensitizers even at a low concentration such as 20μM when megavoltage X-ray is used. Also, when KB cells were treated with F-AuNPs under both laser and X-ray irradiation, the cell viability substantially decreased more than F-AuNPs-enhanced PTT alone or F-AuNPs-enhanced RT alone. Flow cytometry assay clearly indicated that F-AuNPs-mediated photo-thermo-radio therapy significantly induced apoptosis. These results confirm that F-AuNPs is a promising and research-worthy nanoconjugate in the field of targeted photo-thermo-radiotherapy of cancer.
本研究的目的是开发一种优化的方法来制备叶酸偶联金纳米颗粒（F-AuNPs），并研究其在联合光热疗法（PTT）和放射疗法（RT）治疗口腔表皮癌细胞KB时的细胞毒性作用和细胞凋亡情况。为此，用不同浓度的合成F-AuNPs处理细胞6小时，然后用激光束（532nm，0.5W/cm，15分钟）照射。光热疗法后，将细胞暴露于单剂量2Gy的6MV X射线下。进行MTT试验以评估细胞存活率，并使用膜联蛋白V-异硫氰酸荧光素/碘化丙啶凋亡检测试剂盒通过流式细胞术测定细胞凋亡。单独的激光照射或F-AuNPs处理未观察到明显的细胞损伤或细胞凋亡，而用F-AuNPs孵育然后暴露于激光下的细胞活力显著降低。此外，我们的结果表明，当使用兆伏级X射线时，即使在低浓度如20μM时，F-AuNPs也是良好的放射增敏剂。而且，当KB细胞在激光和X射线照射下用F-AuNPs处理时，细胞活力的降低幅度明显大于单独的F-AuNPs增强的PTT或单独的F-AuNPs增强的RT。流式细胞术检测清楚地表明，F-AuNPs介导的光热放射疗法显著诱导细胞凋亡。这些结果证实，F-AuNPs在癌症靶向光热放射治疗领域是一种有前途且值得研究的纳米缀合物。
