Shi Ying, Zhai Qixiao, Li Dongyao, Mao Bingyong, Liu Xiaoming, Zhao Jianxin, Zhang Hao, Chen Wei
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122 Wuxi, PR China; International Joint Research Laboratory for Probiotics, Jiangnan University, 214122 Wuxi, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122 Wuxi, PR China; International Joint Research Laboratory for Probiotics, Jiangnan University, 214122 Wuxi, PR China.
Microbiol Res. 2017 Jul;200:14-24. doi: 10.1016/j.micres.2017.04.001. Epub 2017 Apr 5.
Probiotics have been used to rebuild the antibiotic-induced dysfunction in gut microbiota, but whether the different strains of probiotics result in similar or reverse effects remains unclear. In this study, the different recovery effects of two cocktails (each contains four strains) of Lactobacillus and fructooligosaccharide against cefixime-induced change of gut microbiota were evaluated in C57BL/6J mice. The results show that the use of cefixime caused a reduction in the diversities of the microbial community and led to significantly decreasing to one preponderant Firmicutes phylum, which was difficult to restore naturally in the short term. The gut microbiota compositions of the groups treated with the probiotic cocktails were much more diverse than those of the natural recovery group. The effects of Lactobacillus cocktails against the cefixime-induced gut microbiota change may mainly be due to the beneficial SCFAs production in vivo and also be related to the good cell adhesion properties performed in vitro. Meanwhile, the restoration of the cefixime-induced gut microbiota was significantly different between two Lactobacillus groups since the Lactobacillus strains with high levels of fructooligosaccharide use and better cell adhesion properties performed considerably better than the Lactobacillus strains with high survival rates in the gastrointestinal tract. The contents of short-chain fatty acids in ceca were increased to 26.483±1.925 and 25.609±2.782μmol/g in the two probiotic cocktail groups respectively compared to 15.791±0.833μmol/g (P<0.05) in control group. Moreover, intestinal inflammation was alleviated by administration of the Lactobacillus cocktails. However, fructooligasaccharide administration showed certain effects on gut microbiota restoration (such as an increase of Akkermansia), although its effect on the entire microbiome structure is not so obvious.
益生菌已被用于重建抗生素诱导的肠道微生物群功能障碍，但不同菌株的益生菌是否会产生相似或相反的效果仍不清楚。在本研究中，在C57BL/6J小鼠中评估了两种乳酸菌和低聚果糖混合物（每种包含四种菌株）对头孢克肟诱导的肠道微生物群变化的不同恢复效果。结果表明，使用头孢克肟会导致微生物群落多样性降低，并导致优势菌门厚壁菌门显著减少，短期内难以自然恢复。用益生菌混合物处理的组的肠道微生物群组成比自然恢复组的要多样化得多。乳酸菌混合物对头孢克肟诱导的肠道微生物群变化的影响可能主要归因于体内有益短链脂肪酸的产生，也与体外良好的细胞粘附特性有关。同时，两种乳酸菌组之间头孢克肟诱导的肠道微生物群的恢复存在显著差异，因为低聚果糖利用率高且细胞粘附特性更好的乳酸菌菌株比胃肠道中存活率高的乳酸菌菌株表现要好得多。与对照组的15.791±0.833μmol/g（P<0.05）相比，两个益生菌混合物组盲肠中的短链脂肪酸含量分别增加到26.483±1.925和25.609±2.782μmol/g。此外，给予乳酸菌混合物可减轻肠道炎症。然而，低聚果糖的施用对肠道微生物群的恢复有一定影响（如阿克曼氏菌增加），尽管其对整个微生物组结构的影响不那么明显。
