Shen Haoyi, Zhao Yiran, Zhang Sizhe, Xu Jiahui, Tong Jinjin, Yao Hua, Zhang Hua
Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.
Probiotics Antimicrob Proteins. 2025 May 27. doi: 10.1007/s12602-025-10584-5.
Antibiotic-associated diarrhea (AAD) remains a significant clinical challenge, with current treatments often inadequate for fully restoring gut health. This study aimed to evaluate the effectiveness of synbiotics, including chitosan oligosaccharides, Bifidobacterium, Clostridium butyricum, and Lactiplantibacillus plantarum, in mitigating AAD in a canine model. Sixteen Beagle dogs were randomly assigned to an AAD model group or a synbiotic treatment group. Synbiotic supplementation significantly reduced diarrhea severity, improved intestinal morphology, and enhanced gut microbiota diversity. Furthermore, synbiotics upregulated the expression of tight junction proteins (ZO-1, occludin, claudin-1), thereby reducing mucosal permeability. A significant decrease in proinflammatory cytokines (IL-1β, TNF-α) further underscored the anti-inflammatory effects of the treatment. These findings suggest that synbiotics may offer a promising approach to restoring gut barrier integrity and modulating immune responses in cases of AAD. Further research is warranted to investigate the long-term impacts and underlying mechanisms of synbiotics in gut health restoration.
抗生素相关性腹泻（AAD）仍然是一个重大的临床挑战，目前的治疗方法往往不足以完全恢复肠道健康。本研究旨在评估合生元（包括壳寡糖、双歧杆菌、丁酸梭菌和植物乳杆菌）在减轻犬类模型AAD中的有效性。16只比格犬被随机分配到AAD模型组或合生元治疗组。补充合生元显著降低了腹泻严重程度，改善了肠道形态，并增强了肠道微生物群多样性。此外，合生元上调了紧密连接蛋白（ZO-1、闭合蛋白、Claudin-1）的表达，从而降低了粘膜通透性。促炎细胞因子（IL-1β、TNF-α）的显著降低进一步突出了该治疗的抗炎作用。这些发现表明，合生元可能为恢复AAD病例中的肠道屏障完整性和调节免疫反应提供一种有前景的方法。有必要进一步研究以探讨合生元在肠道健康恢复中的长期影响和潜在机制。
