Müller K M, Fisseler-Eckhoff A
Institute of Pathology, Berufsgenossenschaftlichen Krankenanstalten, Bergmannsheil-University Clinic Bochum, West Germany.
Pathol Res Pract. 1988 Dec;184(1):108-15. doi: 10.1016/S0344-0338(88)80201-2.
The categorization of lung tumors into histologically defined groups and subtypes is based on morphometric criteria, on various degrees of tumoral differentiation and growth patterns. Light microscopic examination of different sections of one and the same tumor reveals in more than 30% of the cases investigated a qualitative and quantitative heterogeneous cell and tumor growth pattern, which is further proved by additional immunohistochemical, electronmicroscopic and DNA-flow cytometry studies. According to observations on the ultrastructural level mixed or combined morphologic features signaling abnormal differentiation patterns in preneoplastic lesions of the bronchial mucosa were visible. So-called amphicrine cells containing mucous vacuoles as well as granules of neurosecretory type were found in basal cell and goblet cell hyperplasia. Squamous-type cells predominate in epidermoid metaplasia and in dysplasia. Cells containing mucous granules or tonofilaments and scant granules of dense core-type suggestive of squamous neurosecretory differentiation were present in all lesions. The ultrastructural features were correlated with the bronchial carcinoma cell heterogeneity and histogenesis.
将肺肿瘤分类为组织学定义的组和亚型是基于形态学标准、肿瘤分化程度和生长模式的不同。对同一肿瘤的不同切片进行光学显微镜检查发现，在超过30%的研究病例中，存在定性和定量的异质性细胞及肿瘤生长模式，这一点通过额外的免疫组织化学、电子显微镜和DNA流式细胞术研究得到了进一步证实。根据超微结构水平的观察，在支气管黏膜的癌前病变中可见混合或组合的形态学特征，表明存在异常分化模式。在基底细胞和杯状细胞增生中发现了所谓的兼性细胞，其含有黏液空泡以及神经分泌型颗粒。在表皮化生和发育异常中，鳞状细胞占主导。在所有病变中均存在含有黏液颗粒或张力丝以及少量致密核心型颗粒的细胞，提示鳞状神经分泌分化。超微结构特征与支气管癌细胞的异质性和组织发生相关。
