Ward J M, Stinson S F, Hardisty J F, Cockrell B Y, Hayden D W
J Natl Cancer Inst. 1979 Apr;62(4):1067-73.
2,4-Diaminoanisole sulfate was fed at dietary levels of 0.12% (low dose) or 0.5% (high dose) to groups of 50 male and 50 female inbred F344 rats for 78 weeks. By 107 weeks after the initial exposure, 58% of the male rats and 42% of the female rats administered the high dose had thyroid neoplasms, whereas only 7--8% of the controls developed them. Follicular cell carcinomas were the primary type of neoplasm induced. None of the controls had these tumors. The carcinomas, which were papillary, cystic, or solid, were highly invasive but did not metastasize. A brown pigment was present as granules primarily in thyroid follicular cells in all exposed rats. The amount of pigment as determined by an image-analyzing computer revealed that the cross-sectional area occupied by the pigment granules and the optical density of the granules were dose related.
将硫酸2,4 -二氨基苯甲醚以0.12%（低剂量）或0.5%（高剂量）的膳食水平投喂给每组50只雄性和50只雌性近交F344大鼠，持续78周。在初次暴露后107周时，接受高剂量的雄性大鼠中有58%、雌性大鼠中有42%发生了甲状腺肿瘤，而对照组中只有7 - 8%发生甲状腺肿瘤。滤泡细胞癌是诱导产生的主要肿瘤类型。对照组均无这些肿瘤。这些癌为乳头状、囊性或实性，具有高度侵袭性但不发生转移。在所有暴露大鼠中，一种棕色色素主要以颗粒形式存在于甲状腺滤泡细胞中。通过图像分析计算机测定的色素量显示，色素颗粒所占的横截面积和颗粒的光密度与剂量相关。
