Carrier D J, Bogri T, Cosentino G P, Guse I, Rakhit S, Singh K
Bio- Méga, Inc., Laval, Québec.
Prostaglandins Leukot Essent Fatty Acids. 1988 Oct;34(1):27-30. doi: 10.1016/0952-3278(88)90021-x.
Alkaline hydrolysis of leukotriene A4 methyl ester to leukotriene A4 was studied in either methanol or acetone. Hydrolysis in acetone yielded larger amounts of leukotriene A4 than similar hydrolysis in methanol. The maximum amount was obtained 60 minutes after the beginning of the hydrolysis. Leukotriene A4, as well as leukotriene B4 methoxy isomers were obtained from hydrolysis of leukotriene A4 methyl ester in methanol. It was found that initial leukotriene A4 methyl ester concentration affected the amount of LTA4 produced during the hydrolysis. The maximum concentration of leukotriene A4 was obtained by hydrolyzing solutions of 0.25 mg/ml leukotriene methyl ester in acetone. Spontaneous degradation of leukotriene A4 occurred when it was diluted with tris buffer. Addition of bovine serum albumin to the tris buffer significantly prolonged the half life of leukotriene A4.
在甲醇或丙酮中研究了白三烯A4甲酯碱水解生成白三烯A4的过程。与在甲醇中进行类似水解相比，在丙酮中水解产生的白三烯A4量更多。水解开始60分钟后获得最大量。白三烯A4以及白三烯B4甲氧基异构体是由白三烯A4甲酯在甲醇中的水解产生的。发现初始白三烯A4甲酯浓度会影响水解过程中产生的LTA4量。通过水解丙酮中0.25mg/ml白三烯甲酯溶液获得白三烯A4的最大浓度。当白三烯A4用tris缓冲液稀释时会发生自发降解。向tris缓冲液中添加牛血清白蛋白可显著延长白三烯A4的半衰期。
