Tang Shi-Lei, Gao Yuan-Lin, Hu Wen-Zhong
Department of Neurosurgery, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, China.
Department of Neurology, Kaifeng Central Hospital, Kaifeng 475000, Henan Province, China.
Biomed Pharmacother. 2017 Aug;92:24-32. doi: 10.1016/j.biopha.2017.05.046. Epub 2017 May 18.
Progestin and AdipoQ Receptor 3 (PAQR3), a member of the PAQR family, is down-regulated in several types of cancers and has been closely associated with tumor progression and development. However, little is known about the functions of PAQR3 in the tumorigenesis of human glioma. Therefore, in this report, we investigated the role of PAQR3 in human glioma. Our results showed that the expression of PAQR3 was significantly reduced in human glioma tissues and cell lines. PAQR3 overexpression inhibited the proliferation of glioma cells in vitro and attenuated tumor xenograft growth in vivo. In addition, PAQR3 overexpression suppressed the migration and invasion of glioma cells, as well as prevented the EMT process. Mechanistic studies demonstrated that PAQR3 overexpression significantly down-regulated the levels of phosphorylated PI3K and Akt in U251 cells. In conclusion, these results demonstrated that PAQR3 inhibited the proliferation, migration and invasion in glioma cells, at least in part, through the inactivation of PI3K/Akt signaling pathway. Therefore, PAQR3 may be a therapeutic target for the treatment of glioma.
孕激素和脂联素受体3（PAQR3）是PAQR家族的成员之一，在多种癌症类型中表达下调，并且与肿瘤进展和发展密切相关。然而，关于PAQR3在人类胶质瘤发生中的功能知之甚少。因此，在本报告中，我们研究了PAQR3在人类胶质瘤中的作用。我们的结果表明，PAQR3在人类胶质瘤组织和细胞系中的表达显著降低。PAQR3过表达在体外抑制胶质瘤细胞的增殖，并在体内减弱肿瘤异种移植的生长。此外，PAQR3过表达抑制胶质瘤细胞的迁移和侵袭，并阻止上皮-间质转化过程。机制研究表明，PAQR3过表达显著下调U251细胞中磷酸化PI3K和Akt的水平。总之，这些结果表明，PAQR3至少部分地通过PI3K/Akt信号通路的失活来抑制胶质瘤细胞的增殖、迁移和侵袭。因此，PAQR3可能是治疗胶质瘤的一个治疗靶点。
