College of Food Science, Sichuan Agricultural University, Yaan 625014, PR China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
College of Food Science, Sichuan Agricultural University, Yaan 625014, PR China.
Int J Biol Macromol. 2017 Oct;103:1130-1137. doi: 10.1016/j.ijbiomac.2017.05.101. Epub 2017 May 18.
Fibrous scaffolds with different ratios of poly (lactic acid) (PLA) and chitosan were fabricated by conventional electrospinning. After crosslinking by the glutaraldehyde vapor, the structure, mechanical properties, hydrophilicity, and in-fiber chemical interactions of the scaffolds were investigated. We found that the fiber diameter decreased with the concentration of chitosan, while mechanical properties and hydrophilicity improved. In addition, we found that scaffolds with aligned fibers have higher mechanical strength and biocompatibility than scaffolds with randomly oriented fibers. In particular, scaffolds with aligned fibers with PLA:chitosan ratios of 7:1 was found to support cardiomyocyte viability, elicit cell elongation, and enhance production of sarcomeric α-actinin and troponin I. Collectively, the data indicate that composite scaffolds consisting of PLA/chitosan fibers have great potential for engineering cardiac tissue, and for accelerating the regeneration of myocardia.
通过常规静电纺丝技术制备了不同比例聚乳酸（PLA）和壳聚糖的纤维支架。用过氧化戊二醛蒸气交联后，对支架的结构、力学性能、亲水性和纤维内化学相互作用进行了研究。结果发现，纤维直径随壳聚糖浓度的增加而减小，而力学性能和亲水性提高。此外，我们发现具有定向纤维的支架比具有随机取向纤维的支架具有更高的机械强度和生物相容性。特别是，具有 PLA:壳聚糖比为 7:1 的定向纤维的支架被发现支持心肌细胞活力，引起细胞伸长，并增强肌节α-肌动蛋白和肌钙蛋白 I 的产生。总的来说，这些数据表明，由 PLA/壳聚糖纤维组成的复合支架在心脏组织工程和加速心肌再生方面具有巨大的潜力。
