Momeni Pegah, Nourisefat Maryam, Farzaneh Arman, Shahrousvand Mohammad, Abdi Mohammad Hossein
Faculty of Polymer Engineering, Sahand University of Technology, Tabriz, Iran.
Department of polymer engineering and color technology, Amirkabir University of Technology, Tehran, Iran.
Heliyon. 2023 Dec 3;10(1):e23218. doi: 10.1016/j.heliyon.2023.e23218. eCollection 2024 Jan 15.
A system based on poly(l-lactic acid) (PLLA) and hydroxypropyl cellulose (HPC) was considered in this study to achieve electrospun mats with outstanding properties and applicability in biomedical engineering. A novel binary solvent system of chloroform/N,N-dimethylformamide (CF/DMF:70/30) was utilized to minimize the probable phase separation between the polymeric components. Moreover, Response Surface Methodology (RSM) was employed to model/optimize the process. Finally, to scrutinize the ability of the complex in terms of drug delivery, Calendula Officinalis (Marigold) extract was added to the solution of the optimal sample (Opt.PH), and then the set was electrospun (PHM). As a result, the presence of Marigold led to higher values of fiber diameter (262 ± 34 nm), pore size (483 ± 102 nm), and surface porosity (81.0 ± 7.3 %). As this drug could also prohibit the micro-scale phase separation, the PHM touched superior tensile strength and Young modulus of 11.3 ± 1.1 and 91.2 ± 4.2 MPa, respectively. Additionally, the cumulative release data demonstrated non-Fickian diffusion with the Korsmeyer-Peppas exponent and diffusion coefficient of n = 0.69 and D = 2.073 × 10 cm/s, respectively. At the end stage, both the Opt.PH and PHM mats manifested satisfactory results regarding the hydrophilicity and cell viability/proliferation assessments, reflecting their high potential to be used in regenerative medicine applications.
本研究考虑了一种基于聚（L-乳酸）（PLLA）和羟丙基纤维素（HPC）的体系，以制备具有优异性能且适用于生物医学工程的电纺垫。采用了一种新型的氯仿/N，N-二甲基甲酰胺二元溶剂体系（CF/DMF：70/30），以尽量减少聚合物组分之间可能的相分离。此外，采用响应面法（RSM）对该过程进行建模/优化。最后，为了考察该复合物在药物递送方面的能力，将金盏花提取物添加到最佳样品（Opt.PH）的溶液中，然后进行电纺（PHM）。结果，金盏花的存在导致纤维直径（262±34nm）、孔径（483±102nm）和表面孔隙率（81.0±7.3%）的值更高。由于这种药物还可以抑制微观相分离，PHM的拉伸强度和杨氏模量分别达到了优异的11.3±1.1和91.2±4.2MPa。此外，累积释放数据表明其为非菲克扩散，Korsmeyer-Peppas指数和扩散系数分别为n = 0.69和D = 2.073×10 cm/s。在最后阶段，Opt.PH和PHM垫在亲水性以及细胞活力/增殖评估方面均表现出令人满意的结果，这反映了它们在再生医学应用中的巨大潜力。
