Tran Huynh Nguyen Khanh, Nguyen Van Thu, Kim Jeong Ah, Rho Seong Soo, Woo Mi Hee, Choi Jae Sui, Lee Jeong-Hyung, Min Byung Sun
College of Pharmacy, Catholic University of Daegu, Gyeongbuk 38430, Republic of Korea.
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Fitoterapia. 2017 Jul;120:17-24. doi: 10.1016/j.fitote.2017.05.004. Epub 2017 May 18.
Five new compounds, 10-oxomornigrol F (1), (7″R)-(-)-6-(7″-hydroxy-3″,8″-dimethyl-2″,8″-octadien-1″-yl)apigenin (2), ramumorin A (3), ramumorin B (4), and (4S,7S,8R)-trihydroxyoctadeca-5Z-enoic acid (5), together with 31 known compounds (6-36), were isolated from the twigs of Morus alba (Moraceae). The chemical structures of these compounds were established using spectroscopic analyses, 1D and 2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and Mosher's methods. The anti-inflammatory activities of the compounds were evaluated by investigating their ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 1, 2, 13, 17, 19, 25-28, and 32 showed inhibitory effects with IC values ranging from 2.2 to 5.3μg/mL. Compounds 1, 2, 17, 25, and 32 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner. In addition, pretreating the cells with compound 1, 17, and 32 significantly suppressed LPS-induced expression of cyclooxygenase-2 (COX-2) protein.
从桑科植物白桑的嫩枝中分离出5种新化合物，即10-氧代桑尼格罗醇F（1）、（7″R）-（-）-6-（7″-羟基-3″,8″-二甲基-2″,8″-辛二烯-1″-基）芹菜素（2）、拉莫莫林A（3）、拉莫莫林B（4）和（4S,7S,8R）-三羟基十八碳-5Z-烯酸（5），以及31种已知化合物（6 - 36）。通过光谱分析、一维和二维核磁共振、高分辨率电喷雾电离质谱（HRESIMS）以及莫舍尔方法确定了这些化合物的化学结构。通过研究化合物抑制巨噬细胞RAW 264.7细胞中脂多糖（LPS）诱导的一氧化氮（NO）产生的能力，评估了它们的抗炎活性。化合物1、2、13、17、19、25 - 28和32表现出抑制作用，IC值范围为2.2至5.3μg/mL。化合物1、2、17、25和32以浓度依赖性方式降低LPS诱导的诱导型一氧化氮合酶（iNOS）表达。此外，用化合物1、17和32预处理细胞可显著抑制LPS诱导的环氧化酶-2（COX-2）蛋白表达。
