核磁共振在膜蛋白研究中的应用。
Applications of NMR to membrane proteins.
作者信息
Opella Stanley J, Marassi Francesca M
机构信息
Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307, USA.
Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Arch Biochem Biophys. 2017 Aug 15;628:92-101. doi: 10.1016/j.abb.2017.05.011. Epub 2017 May 18.
Abstract
Membrane proteins present a challenge for structural biology. In this article, we review some of the recent developments that advance the application of NMR to membrane proteins, with emphasis on structural studies in detergent-free, lipid bilayer samples that resemble the native environment. NMR spectroscopy is not only ideally suited for structure determination of membrane proteins in hydrated lipid bilayer membranes, but also highly complementary to the other principal techniques based on X-ray and electron diffraction. Recent advances in NMR instrumentation, spectroscopic methods, computational methods, and sample preparations are driving exciting new efforts in membrane protein structural biology.
摘要
膜蛋白给结构生物学带来了挑战。在本文中，我们回顾了一些推动核磁共振（NMR）技术应用于膜蛋白研究的最新进展，重点是在类似于天然环境的无去污剂脂质双层样品中的结构研究。核磁共振光谱不仅非常适合于确定水合脂质双层膜中膜蛋白的结构，而且与基于X射线和电子衍射的其他主要技术具有高度互补性。NMR仪器、光谱方法、计算方法和样品制备方面的最新进展正在推动膜蛋白结构生物学领域令人兴奋的新研究。
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