Kishimoto Takuya Evan, Yashima Shoko, Nakahira Rei, Onozawa Eri, Azakami Daigo, Ujike Makoto, Ochiai Kazuhiko, Ishiwata Toshiyuki, Takahashi Kimimasa, Michishita Masaki
Department of Veterinary Pathology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
J Vet Med Sci. 2017 Jul 7;79(7):1155-1162. doi: 10.1292/jvms.16-0412. Epub 2017 May 21.
Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres derived from canine rhabdomyosarcoma cells have stem cell-like properties is unclear. We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres in vitro and in vivo. Sphere-forming cells were more resistant to vincristine, mitoxantrone and doxorubicin than adherent cells. Xenograft transplantation demonstrated that 1 × 10 sphere-forming cells derived from CMS-C were sufficient for tumor formation. The sphere assay showed that the sphere-forming cells were present in these tumors. These results suggest that the spheres derived from canine rhabdomyosarcoma cells may possess characteristics of TICs. This study provides the foundation for elucidating the contribution of TICs to rhabdomyosarcoma tumorigenesis.
癌症干细胞或肿瘤起始细胞（TICs）是一小部分具有自我更新、分化和引发肿瘤能力的细胞亚群。这些细胞可能在肿瘤起始、侵袭和复发中发挥作用。源自犬横纹肌肉瘤细胞的球体是否具有干细胞样特性尚不清楚。我们在犬横纹肌肉瘤细胞系CMS - C和CMS - J中诱导形成球体，并在体外和体内对这些球体进行表征。成球细胞比贴壁细胞对长春新碱、米托蒽醌和阿霉素更具抗性。异种移植实验表明，源自CMS - C的1×10个成球细胞足以形成肿瘤。球体分析显示这些肿瘤中存在成球细胞。这些结果表明，源自犬横纹肌肉瘤细胞的球体可能具有肿瘤起始细胞的特征。本研究为阐明肿瘤起始细胞对横纹肌肉瘤肿瘤发生的贡献奠定了基础。
