Quesenberry P, Goldberg L
Department of Medicine, Brown University/Rhode Island Hospital, Providence, RI, USA.
Leukemia. 2017 Aug;31(8):1678-1685. doi: 10.1038/leu.2017.155. Epub 2017 May 22.
The adult marrow hematopoietic stem cell biology has largely been based on studies of highly purified stem cells. This is unfortunate because during the stem cell purification the great bulk of stem cells are discarded. These cells are actively proliferating. The final purified stem cell is dormant and not representative of the whole stem cell compartment. Thus, a large number of studies on the cellular characteristics, regulators and molecular details of stem cells have been carried on out of non-represented cells. Niche studies have largely pursued using these purified stem cells and these are largely un-interpretable. Other considerations include the distinction between baseline and transplant stem cells and the modulation of stem cell phenotype by extracellular vesicles, to cite a non-inclusive list. Work needs to proceed on characterizing the true stem cell population.
成体骨髓造血干细胞生物学在很大程度上是基于对高度纯化干细胞的研究。这很不幸，因为在干细胞纯化过程中，大量的干细胞被丢弃了。这些细胞处于活跃增殖状态。最终纯化的干细胞是休眠的，并不代表整个干细胞群体。因此，大量关于干细胞的细胞特征、调节因子和分子细节的研究是在不具代表性的细胞上进行的。生态位研究在很大程度上使用这些纯化的干细胞，而这些研究在很大程度上是无法解释的。其他需要考虑的因素包括基线干细胞和移植干细胞之间的区别，以及细胞外囊泡对干细胞表型的调节，这只是一个不完整的列举。需要继续开展工作来鉴定真正的干细胞群体。
