Li Xisheng, Yan Jun, Fan Rong, Luo Bin, Zhang Qingmei, Lin Yongda, Zhou Sufang, Luo Guorong, Xie Xiaoxun, Xiao Shaowen
Department of Neurosurgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Oncol Lett. 2017 May;13(5):3080-3086. doi: 10.3892/ol.2017.5799. Epub 2017 Mar 3.
OY-TES-1 is a member of the cancer/testis antigen family that is expressed in healthy testis tissue and certain types of cancerous tissue. The present study aimed to analyze the expression pattern of OY-TES-1 and serum anti-OY-TES-1 antibody concentration in patients with glioma. OY-TES-1 mRNA was detected in 28/36 (78%) of glioma cases using conventional reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-quantitative-PCR revealed that OY-TES-1 was expressed at a higher level in glioma tissues compared with normal adult tissues (with the exception of testis tissue). Anti-OY-TES-1 antibodies were present in the serum of 5/36 (14%) of patients with glioma, but absent in all the serum samples from 107 healthy donors. Immunohistochemical analysis demonstrated that OY-TES-1 protein was expressed in all glioma tissues from patients with anti-OY-TES-1 antibody seropositivity. These results suggest that OY-TES-1 is a novel candidate for glioma immunotherapy.
OY-TES-1是癌症/睾丸抗原家族的成员，在健康的睾丸组织和某些类型的癌组织中表达。本研究旨在分析胶质瘤患者中OY-TES-1的表达模式及血清抗OY-TES-1抗体浓度。使用传统逆转录聚合酶链反应（RT-PCR）分析在36例胶质瘤病例中的28例（78%）检测到OY-TES-1 mRNA。逆转录定量PCR显示，与正常成人组织（睾丸组织除外）相比，OY-TES-1在胶质瘤组织中表达水平更高。36例胶质瘤患者中有5例（14%）血清中存在抗OY-TES-1抗体，但107名健康供者的所有血清样本中均未检测到。免疫组织化学分析表明，抗OY-TES-1抗体血清阳性患者的所有胶质瘤组织中均表达OY-TES-1蛋白。这些结果表明，OY-TES-1是胶质瘤免疫治疗的一个新候选物。
