FMR1NB 参与脑肿瘤发生，是预后和治疗的有希望的靶点。
FMR1NB Involved in Glioma Tumorigenesis Is a Promising Target for Prognosis and Therapy.
机构信息
Department of Neurosurgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, 530021, China.
出版信息
Curr Med Sci. 2022 Aug;42(4):803-816. doi: 10.1007/s11596-022-2586-4. Epub 2022 Jul 11.
OBJECTIVE
Cancer/testis antigen FMR1NB is aberrantly expressed in various types of cancer, but not in normal tissues except for testis. This study aimed to investigate the expression and functional role of FMR1NB in glioma.
METHODS
The expression of FMR1NB mRNA and protein was determined using RT-PCR and immunohistochemistry, respectively, in glioma specimens from 83 patients at follow-up. The effects of siRNA-mediated FMR1NB silencing on malignant biological behaviors were evaluated in glioma cell lines A172 and U251.
RESULTS
FMR1NB mRNA and protein expression was detected in 58.8% (77/131) and 46.34% (57/123) of glioma tissues, respectively. FMR1NB protein was positively correlated with World Health Organization grade and found to be an independent prognostic marker for poor outcome. Knockdown of FMR1NB induced apoptosis and suppressed proliferation, adhesion, migration, and invasion by modulating the expression of cyclin A, CDK2, caspase-3, E-cadherin, and N-cadherin in A172 and U251 cells.
CONCLUSION
Our findings suggest that FMR1NB contributes to the tumorigenesis of glioma cells and may represent a potential prognostic biomarker and an attractive therapeutic target in glioma.
目的
癌/睾丸抗原 FMR1NB 在多种类型的癌症中异常表达，但除睾丸外，在正常组织中不表达。本研究旨在探讨 FMR1NB 在神经胶质瘤中的表达及功能作用。
方法
采用 RT-PCR 和免疫组织化学法分别检测 83 例随访神经胶质瘤标本中 FMR1NBmRNA 和蛋白的表达。用 siRNA 介导的 FMR1NB 沉默评估其对神经胶质瘤细胞系 A172 和 U251 中恶性生物学行为的影响。
结果
FMR1NBmRNA 和蛋白分别在 58.8%（77/131）和 46.34%（57/123）的神经胶质瘤组织中表达。FMR1NB 蛋白与世界卫生组织分级呈正相关，是预后不良的独立预后标志物。FMR1NB 敲低通过调节 A172 和 U251 细胞中环蛋白 A、CDK2、半胱氨酸天冬氨酸蛋白酶-3、E-钙黏蛋白和 N-钙黏蛋白的表达，诱导细胞凋亡，抑制增殖、黏附、迁移和侵袭。
结论
我们的研究结果表明，FMR1NB 有助于神经胶质瘤细胞的肿瘤发生，可能是神经胶质瘤的一个有前途的预后生物标志物和治疗靶点。
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