Chaturvedi Pavan, Rodriguez Stacy D, Vlassiouk Ivan, Hansen Immo A, Smirnov Sergei N
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, United States.
Department of Physics, New Mexico State University, Las Cruces, New Mexico 88003, United States.
ACS Sens. 2016 May 27;1(5):488-492. doi: 10.1021/acssensors.6b00003. Epub 2016 Mar 8.
A simple sensor for viral particles based on ionic conductivity through anodized alumina membranes was demonstrated using MS2 bacteriophage as an example. A facile two-point measuring scheme is geared toward realization using a computer's sound card input/output capabilities suitable for a fast and inexpensive point of care testing. The lowest detection concentration down to ~7 pfu/mL and a large dynamic range up to ~2000 pfu/mL were obtained due to physical optimization that included proper length and diameter for the pores, removing the oxide layer at the electrode, as well as the chemical optimization of covalent binding of antibodies to the pore's walls.
以MS2噬菌体为例，展示了一种基于通过阳极氧化铝膜的离子电导率的病毒颗粒简易传感器。一种简便的两点测量方案旨在利用计算机声卡的输入/输出功能来实现，适用于快速且低成本的即时检测。通过物理优化，包括确定合适的孔长度和直径、去除电极处的氧化层以及对抗体与孔壁共价结合进行化学优化，获得了低至约7 pfu/mL的最低检测浓度和高达约2000 pfu/mL的大动态范围。
