Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
Bioelectrochemistry. 2012 Dec;88:15-21. doi: 10.1016/j.bioelechem.2012.04.006. Epub 2012 May 2.
The Faradaic electrochemical impedance technique is employed to characterize the impedance change of a nanoporous alumina biosensor in response towards the specific binding of dengue serotype 2 (Denv2) viral particles to its serotype 2-specific immunoglobulin G antibody within the thin alumina layer. The optimal equivalent circuit model that matches the impedimetric responses of the sensor describes three distinct regions: the electrolyte solution (R(s)), the porous alumina channels (including biomaterials) (Q(1), R(1)) and the conductive electrode substrate layer (Q(2), R(2)). Both channel resistance R(1) and capacitance Q(1) change in response to the increase of the Denv2 virus concentration. A linear relationship between R(1) and Denv2 concentration from 1 to 900 plaque forming unit per mL (pfu mL(-1)) can be derived using Langmuir-Freundlich isotherm model. At 1pfu mL(-1) Denv2 concentration, R(1) can be distinguished from that of the cell culture control sample. Moreover, Q(1) doubles when Denv2 is added but remains unchanged in the presence of two other non-specific viruses - West Nile virus and Chikungunya virus indicates biosensor specificity can be quantitatively measured using channel capacitance.
电化学交流阻抗技术用于描述纳米多孔氧化铝生物传感器的阻抗变化，该传感器响应登革热血清型 2（Denv2）病毒颗粒与薄氧化铝层内针对其血清型 2 特异性免疫球蛋白 G 抗体的特异性结合。与传感器的阻抗响应匹配的最佳等效电路模型描述了三个不同的区域：电解质溶液（R(s)）、多孔氧化铝通道（包括生物材料）（Q(1), R(1)）和导电电极衬底层（Q(2), R(2)）。通道电阻 R(1)和电容 Q(1)都随 Denv2 病毒浓度的增加而变化。可以使用 Langmuir-Freundlich 等温模型从 1 到 900 噬菌斑形成单位每毫升（pfu mL(-1)) 的 Denv2 浓度导出 R(1) 与 Denv2 浓度之间的线性关系。在 1 pfu mL(-1) Denv2 浓度下，R(1)可以与细胞培养对照样品区分开来。此外，当添加 Denv2 时 Q(1)增加一倍，但在存在另外两种非特异性病毒 - 西尼罗河病毒和基孔肯雅病毒时保持不变，这表明可以使用通道电容定量测量生物传感器的特异性。
