俄亥俄野生动物保护中心及其周边地区采集的粪便中DNA的检测。
Detection of DNA in feces collected in and around an Ohio Wildlife Conservation Center.
作者信息
Sinnott D, Torres K Moreno, Wolfe B, Garabed R, Marsh A E
机构信息
Department of Veterinary Preventive Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH 43210, USA.
Morris Animal Foundation, 720 S. Colorado Blvd., Suite 174A Denver, CO 80246, USA.
出版信息
Vet Parasitol Reg Stud Reports. 2016 Dec;6:31-34. doi: 10.1016/j.vprsr.2016.08.003. Epub 2016 Aug 27.
This study aimed to identify DNA attributed to oocysts in the feces of wild canids in and around an Ohio wildlife conservation center. Two hundred and eighty-five wild canid fecal samples were analyzed using PCR with melting curve analysis to detect coccidian DNA. Coccidia-positive samples were further subjected to -specific and -specific PCR assays. Samples positive by the -specific assay were additionally analyzed with a PCR assay to distinguish from . Coccidian DNA was detected in 51 of the 285 (17.9%) wildlife samples. DNA was detected in three of the coccidia-positive wildlife samples (1.1%) and was detected in none. Determining the presence of in wild canids will contribute to a greater understanding of the role these hosts play in the ecology of this parasite.
本研究旨在鉴定俄亥俄州野生动物保护中心及其周边野生犬科动物粪便中卵囊的DNA。使用带有熔解曲线分析的聚合酶链反应（PCR）对285份野生犬科动物粪便样本进行分析，以检测球虫DNA。对球虫阳性样本进一步进行特异性和特异性PCR检测。通过特异性检测呈阳性的样本，另外用PCR检测进行分析，以区分与。在285份野生动物样本中的51份（17.9%）检测到球虫DNA。在3份球虫阳性野生动物样本（1.1%）中检测到DNA，未检测到。确定野生犬科动物中是否存在将有助于更深入了解这些宿主在这种寄生虫生态学中所起的作用。
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