Ali Attarad, Haq Ihsan Ul, Akhtar Javeed, Sher Muhammad, Ahmed Naveed, Zia Muhammad
Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan.
IET Nanobiotechnol. 2017 Jun;11(4):477-484. doi: 10.1049/iet-nbt.2016.0086.
Cellulose is the natural biopolymer normally used as supporting agent with enhanced applicability and properties. In present study, cellulose isolated from citrus waste is used for silver nanoparticles (Ag-NPs) impregnation by a simple and reproducible method. The Ag-NPs fabricated cellulose (Ag-Cel) was characterised by powder X-rays diffraction, Fortier transform infrared spectroscopy and scanning electron microscopy. The thermal stability was studied by thermo-gravimetric analysis. The antibacterial activity performed by disc diffusion assay reveals good zone of inhibition against and by Ag-Cel as compared Ag-NPs. The discs also displayed more than 90% reduction of culture in broth within 150 min. The Ag-Cel discs also demonstrated minor 2,2-diphenyl 1-picryl-hydrazyl radical scavenging activity and total reducing power ability while moderate total antioxidant potential was observed. Ag-Cel effectively degrades methylene-blue dye up to 63.16% under sunlight irradiation in limited exposure time of 60 min. The Ag-NPs impregnated cellulose can be effectively used in wound dressing to prevent bacterial attack and scavenger of free radicals at wound site, and also as filters for bioremediation and wastewater purification.
纤维素是一种天然生物聚合物,通常用作具有增强适用性和性能的支撑剂。在本研究中,通过一种简单且可重复的方法,将从柑橘废料中分离出的纤维素用于浸渍银纳米颗粒(Ag-NPs)。通过粉末X射线衍射、傅里叶变换红外光谱和扫描电子显微镜对制备的Ag-NPs纤维素(Ag-Cel)进行了表征。通过热重分析研究了其热稳定性。通过纸片扩散法进行的抗菌活性研究表明,与Ag-NPs相比,Ag-Cel对[具体细菌名称1]和[具体细菌名称2]具有良好的抑菌圈。这些纸片在150分钟内还使肉汤中的[具体细菌名称3]培养物减少了90%以上。Ag-Cel纸片还表现出轻微的2,2-二苯基-1-苦基肼自由基清除活性和总还原能力,同时观察到适度的总抗氧化潜力。在60分钟的有限光照时间内,Ag-Cel在阳光照射下能有效降解亚甲基蓝染料,降解率高达63.16%。浸渍了Ag-NPs的纤维素可有效地用于伤口敷料,以防止伤口部位受到细菌侵袭和清除自由基,还可作为生物修复和废水净化的过滤器。