College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071000, China.
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071000, China.
Food Chem. 2017 Oct 15;233:422-428. doi: 10.1016/j.foodchem.2017.04.146. Epub 2017 Apr 26.
The 3D structures of two dummy templates and four phenothiazine drugs were studied by using computational simulation method. Then the two dummy templates were used to synthesize two molecularly imprinted polymers respectively. Results showed that the recognition abilities of the two polymers were consistent with the theoretical calculation. Then a solid phase extraction column was developed for extraction of the four phenothiazines in meat (pork, chicken) followed by determination with high performance liquid chromatography. The column showed high adsorption capacities (850-962ng analyte per milligram of polymer) and high recoveries (93-98%) to the four drugs, and could be recycled for sixty times. The limits of detection were in the range of 1.0-10ng/g, and the recoveries from the fortified blank samples were in the range of 70.3-96.1%. This is the first study reporting the use of molecularly imprinted polymer-based method for determination of phenothiazines residues in foods.
采用计算模拟的方法研究了两个虚拟模板和四个吩噻嗪类药物的三维结构，然后分别用这两个虚拟模板合成了两种分子印迹聚合物。结果表明，两种聚合物的识别能力与理论计算相符。随后，开发了一种固相萃取柱，用于萃取肉（猪肉、鸡肉）中的四种吩噻嗪类药物，然后用高效液相色谱法进行测定。该柱对四种药物具有较高的吸附容量（每毫克聚合物 850-962ng 分析物）和较高的回收率（93-98%），可回收 60 次。检测限范围为 1.0-10ng/g，加标空白样品的回收率范围为 70.3-96.1%。这是首次报道使用基于分子印迹聚合物的方法测定食品中吩噻嗪类药物残留量。
