Jia Bing-Jie, Huang Jun, Liu Ju-Xiang, Wang Jian-Ping
a College of Veterinary Medicine , Hebei Agricultural University , Baoding , Hebei , China.
b Continuing Education College , Hebei Agricultural University , Baoding , Hebei , China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Jan;36(1):74-83. doi: 10.1080/19440049.2018.1562238. Epub 2019 Jan 8.
In this study, 4-nitrotoluene (NT) was used as dummy template to synthesize a molecularly imprinted polymer that was highly specific for chloramphenicol. The polymer was coated in the wells of 96-well microplates as recognition reagent to develop a chemiluminescence method. The analyte solution and an enzyme-labelled hapten were added into the wells to perform competition, and the light signal was induced with a highly efficient luminol-HO-4-(imidazol-1-yl)phenol system. Then, the optimized method was used to determine chloramphenicol in meat (chicken, pork and fish), and the limit of detection (LOD) was 5.0 pg g. Furthermore, the polymer-coated plate could be reused four times, and one test could be finished within 20 min. The recoveries from the standard fortified blank meat samples were in the range of 71.5-94.4%. Therefore, this method could be used as a useful tool for routine screening the residue of chloramphenicol in meat samples.
在本研究中，以4-硝基甲苯（NT）作为虚拟模板来合成对氯霉素具有高度特异性的分子印迹聚合物。该聚合物被涂覆在96孔微孔板的孔中作为识别试剂，以开发一种化学发光方法。将分析物溶液和酶标记的半抗原加入孔中进行竞争反应，并用高效的鲁米诺-HO-4-（咪唑-1-基）苯酚体系诱导光信号。然后，使用优化后的方法测定肉类（鸡肉、猪肉和鱼肉）中的氯霉素，检测限（LOD）为5.0 pg/g。此外，涂有聚合物的微孔板可重复使用4次，一次检测可在20分钟内完成。标准添加空白肉类样品的回收率在71.5%-94.4%范围内。因此，该方法可作为常规筛查肉类样品中氯霉素残留的有用工具。
