Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, People's Republic of China.
Mikrochim Acta. 2017 Dec 11;185(1):46. doi: 10.1007/s00604-017-2608-2.
D-Glucose was used as a dummy template in a strategy to fabricate boronate-based hollow molecularly imprinted polymers (h-MIPs) for the recognition of glycosides. 3-Aminophenylboronic acid (APBA) was selected as the functional monomer to impart glycoside binding capability. A polystyrene core was synthesized via an emulsifier-free emulsion polymerization. It was then be corroded to form a hollow structure where the binding sites on the inner surface can be fully utilized. Adsorption studies indicate that the resulting h-MIPs can specifically recognize glycosides. The h-MIPs were applied as a sorbent for solid phase extraction of the glycosides (daidzin, glycitin, rutin, and genistin) in soy products. Following desorption with 1% TFA in acetonitrile-water (3:7, v/v), the glycosides were quantified by HPLC with UV detection. The detection limits for the glycosides range from 3.5 to 10.8 ng·mL. Graphical abstract Boronate-affinity hollow molecularly imprinted polymers using D-glucose as dummy template were prepared as solid phase extraction adsorbents for selective enrichment of glycosides in soy products prior to quantitation by HPLC-UV.
D-葡萄糖被用作一种虚拟模板，用于制造基于硼酸的中空分子印迹聚合物（h-MIPs），以识别糖苷。3-氨基苯硼酸（APBA）被选为功能单体，赋予糖苷结合能力。通过无乳化剂乳液聚合合成聚苯乙烯核。然后将其腐蚀以形成中空结构，从而可以充分利用内表面上的结合位点。吸附研究表明，所得的 h-MIPs 可以特异性识别糖苷。将 h-MIPs 用作固相萃取大豆制品中糖苷（大豆苷、大豆苷元、芦丁和染料木苷）的吸附剂。用乙腈-水（3:7，v/v）中的 1% TFA 洗脱后，通过 HPLC-UV 检测定量糖苷。糖苷的检测限范围为 3.5 至 10.8 ng·mL。摘要 以 D-葡萄糖为虚拟模板的硼酸亲和中空分子印迹聚合物被制备为固相萃取吸附剂，用于在通过 HPLC-UV 定量之前选择性富集大豆制品中的糖苷。
