Almada M, Leal-Martínez B H, Hassan N, Kogan M J, Burboa M G, Topete A, Valdez M A, Juárez J
Departamento de Física, Universidad de Sonora, Hermosillo, Sonora 83000, Mexico.
Programa Institucional de Fomento a la Investigación, Desarrollo e Innovación, Universidad Tecnológica Metropolitana (UTEM), Chile.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:583-593. doi: 10.1016/j.msec.2017.03.218. Epub 2017 Mar 30.
Gold nanorods (GNR) use has been proposed in medical applications because of their intrinsic photothermal properties. However, the presence of CTAB molecules adsorbed onto the surface of GNRs results in a highly cytotoxic GNR system. In this work we replace the CTAB molecules with a thiolated chitosan. Once chitosan coated GNRs (Chi-SH-GNR) were attained, a film of alginate (Alg-Chi-SH-GNR) or polyvinyl alcohol (PVA-Chi-SH-GNR) was deposited onto the surface of Chi-GNR by a layer-by-layer process. The photothermal conversion efficiency for the GNR systems was determined irradiating the GNRs suspended in aqua media with a CW 808nm diode laser (CNI, China). The cytotoxicity effect and the photothermal cellular damage of GNR systems were evaluated on a breast cancer cell line. Results show that polymer coats did not affect the transduction photothermal efficiency. Values around 50% were obtained for the different coated gold nanorods. The cytotoxicity of coated gold nanorods diminished significantly compared with those GNR stabilized with CTAB. The laser irradiation of cells treated with gold nanorods showed a decrease in their viability compared with the cells treated but no irradiated.
由于金纳米棒（GNR）具有固有的光热特性，其在医学应用中的使用已被提出。然而，吸附在GNR表面的CTAB分子的存在导致了一个具有高度细胞毒性的GNR系统。在这项工作中，我们用硫醇化壳聚糖取代了CTAB分子。一旦获得了壳聚糖包覆的GNR（Chi-SH-GNR），就通过层层组装工艺在Chi-GNR表面沉积一层藻酸盐（Alg-Chi-SH-GNR）或聚乙烯醇（PVA-Chi-SH-GNR）薄膜。通过用连续波808nm二极管激光器（中国长春新产业光电技术有限公司）照射悬浮在水介质中的GNR来测定GNR系统的光热转换效率。在一种乳腺癌细胞系上评估了GNR系统的细胞毒性作用和光热细胞损伤。结果表明，聚合物涂层不影响光热转换效率。不同包覆的金纳米棒的光热转换效率值约为50%。与用CTAB稳定的GNR相比，包覆金纳米棒的细胞毒性显著降低。与未接受激光照射的处理细胞相比，用金纳米棒处理的细胞经激光照射后活力下降。
