Stastna Miroslava, Gottlieb Roberta A, Van Eyk Jennifer E
a Heart Institute , Cedars-Sinai Medical Center , Los Angeles , CA , USA.
b Advanced Clinical BioSystems Research Institute , Cedars-Sinai Medical Center , Los Angeles , CA , USA.
Expert Rev Proteomics. 2017 Jun;14(6):529-543. doi: 10.1080/14789450.2017.1333424. Epub 2017 May 26.
Protein synthesis is the outcome of tightly regulated gene expression which is responsive to a variety of conditions. Efforts are ongoing to monitor individual stages of protein synthesis to ensure maximum efficiency and accuracy. Due to post-transcriptional regulation mechanisms, the correlation between translatome and proteome is higher than between transcriptome and proteome. However, the most accurate approach to assess the key modulators and final protein expression is directly by using proteomics. Areas covered: This review covers various proteomic strategies that were used to better understand post-transcriptional regulation, specifically during and early after translation. The methods that identify both regulatory proteins associated with translational components and newly synthesized proteins are discussed. Expert commentary: Emerging proteomic approaches make it possible to monitor protein dynamics in cells, tissues and whole animals. The ability to detect alteration in protein abundance soon after their synthesis enables earlier recognition of disease causing factors and candidates to prevent/rectify disease phenotype.
蛋白质合成是受到严格调控的基因表达的结果，该过程对多种条件都有响应。目前正在努力监测蛋白质合成的各个阶段，以确保最高效率和准确性。由于存在转录后调控机制，翻译组与蛋白质组之间的相关性高于转录组与蛋白质组之间的相关性。然而，评估关键调节因子和最终蛋白质表达的最准确方法是直接使用蛋白质组学。涵盖领域：本综述涵盖了各种蛋白质组学策略，这些策略用于更好地理解转录后调控，特别是在翻译过程中及翻译后早期。文中讨论了鉴定与翻译组件相关的调节蛋白和新合成蛋白的方法。专家评论：新兴的蛋白质组学方法使监测细胞、组织和整个动物中的蛋白质动态成为可能。在蛋白质合成后不久就能检测到其丰度变化的能力，有助于更早地识别致病因素以及预防/纠正疾病表型的候选因素。
