Patlaka Christina, Mira Pascual Laia, Paulie Staffan, Henriksson Anni-Frid, Arner Peter, Lång Pernilla, Andersson Göran
a Division of Pathology, Department of Laboratory Medicine , Karolinska Institutet, Karolinska University Hospital Huddinge , Stockholm , Sweden.
b Mabtech AB , Nacka Strand , Sweden.
Biomarkers. 2017 Dec;22(8):764-774. doi: 10.1080/1354750X.2017.1334155. Epub 2017 Jun 8.
Tartrate-resistant acid phosphatase (TRAP) exists as two isoforms, 5a and 5b. TRAP 5a is elevated in adipose tissue of obese women, interacts with pre-adipocytes and is linked to insulin-sensitive hyperplastic obesity when overexpressed in mice. The aim of this study was to investigate the relation between serum TRAP 5a, adiposity indices and metabolic syndrome risk markers in lean and obese women, using a newly developed TRAP 5a-specific ELISA.
A TRAP 5a sandwich ELISA was optimized using TRAP 5a-specific monoclonal antibodies and tested in sera of healthy males. TRAP 5a levels were quantitated in sera from healthy males and lean and obese women.
Serum TRAP 5a protein levels were lower in obese women in comparison with lean. In obese, but not in lean women, serum TRAP 5a correlated positively to % fat mass, BMI, waist- and hip circumference, waist-to-hip ratio and PAI, while no correlations to serum leptin, HOMA, glucose, insulin, FFA, HDL, TG, APO-A1 and APO-B were observed.
TRAP 5a serum levels correlated positively to anthropometric obesity parameters but not to metabolic syndrome risk factors, indicating that serum TRAP 5a is associated with pathological adipose tissue expansion.
抗酒石酸酸性磷酸酶（TRAP）以5a和5b两种同工型存在。TRAP 5a在肥胖女性的脂肪组织中升高，与前脂肪细胞相互作用，在小鼠中过表达时与胰岛素敏感的增生性肥胖有关。本研究的目的是使用新开发的TRAP 5a特异性酶联免疫吸附测定法（ELISA），研究瘦女性和肥胖女性血清TRAP 5a、肥胖指数与代谢综合征风险标志物之间的关系。
使用TRAP 5a特异性单克隆抗体优化TRAP 5a夹心ELISA，并在健康男性血清中进行测试。对健康男性以及瘦女性和肥胖女性的血清中TRAP 5a水平进行定量。
与瘦女性相比，肥胖女性血清TRAP 5a蛋白水平较低。在肥胖女性而非瘦女性中，血清TRAP 5a与体脂百分比、体重指数、腰围和臀围、腰臀比及纤溶酶原激活物抑制剂（PAI）呈正相关，而与血清瘦素、稳态模型评估胰岛素抵抗指数（HOMA）、葡萄糖、胰岛素、游离脂肪酸（FFA）、高密度脂蛋白（HDL）、甘油三酯（TG）、载脂蛋白A1（APO-A1）和载脂蛋白B（APO-B）无相关性。
TRAP 5a血清水平与人体测量学肥胖参数呈正相关，但与代谢综合征风险因素无关，表明血清TRAP 5a与病理性脂肪组织扩张有关。
