Kylmäoja Elina, Nakamura Miho, Turunen Sanna, Patlaka Christina, Andersson Göran, Lehenkari Petri, Tuukkanen Juha
Institute of Cancer Research and Translational Medicine, Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, P.O. Box 5000, 90014, Finland.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan.
Heliyon. 2018 Sep 12;4(9):e00780. doi: 10.1016/j.heliyon.2018.e00780. eCollection 2018 Sep.
Bone marrow (BM) and peripheral blood (PB) derived mononuclear cells are precursors of osteoclast differentiation. However, few studies have compared the phenotypic and functional properties of osteoclasts generated from these sources and the effects of different growth factors on osteoclastogenesis. Both cell types differentiated into functional osteoclasts, but culturing the cells with or without transforming growth factor beta (TGF-β) and dexamethasone revealed differences in their osteoclastogenic capacity. When receptor activator for nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were used for differentiation, we did not observe differences in bone resorption activity or expression of osteoclastogenic genes () and () between the osteoclasts formed from the two sources. Addition of TGF-β and dexamethasone led to higher number of nuclei in multinuclear cells and increased expression of tartrate resistant acid phosphatase (TRACP) 5a and 5b, and in PB- derived osteoclasts depicting the higher osteoclastogenic potential and responsiveness to TGF-β and dexamethasone in PB monocytes. These results conclude that the choice of the osteoclast precursor source as well as the choice of osteoclastogenic growth factors are essential matters in determining the phenotypic characteristics of heterogeneous osteoclast populations.
骨髓（BM）和外周血（PB）来源的单核细胞是破骨细胞分化的前体。然而，很少有研究比较过由这些来源产生的破骨细胞的表型和功能特性，以及不同生长因子对破骨细胞生成的影响。两种细胞类型都分化为功能性破骨细胞，但在有或没有转化生长因子β（TGF-β）和地塞米松的情况下培养细胞，揭示了它们在破骨细胞生成能力上的差异。当使用核因子κB受体活化因子配体（RANKL）和巨噬细胞集落刺激因子（M-CSF）进行分化时，我们没有观察到由这两种来源形成的破骨细胞在骨吸收活性或破骨细胞生成基因（）和（）的表达上存在差异。添加TGF-β和地塞米松导致多核细胞中的细胞核数量增加，并且在PB来源的破骨细胞中抗酒石酸酸性磷酸酶（TRACP）5a和5b、以及（此处原文似乎缺失部分内容）的表达增加，这表明PB单核细胞中破骨细胞生成潜力更高，对TGF-β和地塞米松的反应性更强。这些结果表明，破骨细胞前体来源的选择以及破骨细胞生成生长因子的选择，对于确定异质性破骨细胞群体的表型特征至关重要。
