National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Trends Plant Sci. 2017 Jul;22(7):550-553. doi: 10.1016/j.tplants.2017.05.001. Epub 2017 May 19.
Clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated proteins (CRISPR-Cas), a groundbreaking genome-engineering tool, has facilitated targeted trait improvement in plants. Recently, CRISPR-CRISPR from Prevotella and Francisella 1 (Cpf1) has emerged as a new tool for efficient genome editing, including DNA-free editing in plants, with higher efficiency, specificity, and potentially wider applications than CRISPR-Cas9.
成簇规律间隔短回文重复序列 (CRISPR)-CRISPR 相关蛋白 (CRISPR-Cas) 是一种开创性的基因组工程工具,可促进植物的靶向性状改良。最近,源自普雷沃氏菌和弗朗西斯菌 1 (Cpf1) 的 CRISPR-CRISPR 成为一种新的基因组编辑工具,包括在植物中进行无 DNA 编辑,其效率、特异性更高,潜在应用范围更广,优于 CRISPR-Cas9。