[控制对甲氧苄啶和链丝菌素耐药性的Tn7样转座子插入大肠杆菌染色体的定位]
[Localization of insertion into E. coli chromosome of Tn7-like transposons controlling resistance to trimethoprim and streptothricin].
作者信息
Tietze E, Golubev A V, Shovadaeva G A
出版信息
Mol Gen Mikrobiol Virusol. 1988 Nov(11):18-20.
PMID:2853300
Abstract
To localize the insertion sites for Tn7-like transposons Tn1824, Tn1825 and Tn1826 the EcoRI-cleaved fragments of E. coli recA+ and recA- strains chromosome carrying the transposons were hybridized. It was shown that transposition of Tn7-like elements takes place in the strictly defined region of E. coli chromosome, like it had been previously described for transposon Tn7.
摘要
为了定位类Tn7转座子Tn1824、Tn1825和Tn1826的插入位点，将携带这些转座子的大肠杆菌recA⁺和recA⁻菌株染色体的EcoRI酶切片段进行杂交。结果表明，类Tn7元件的转座发生在大肠杆菌染色体的严格限定区域，这与之前对转座子Tn7的描述一致。
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