[转座子Tn2555的蔗糖利用决定因素]
[Sucrose utilization determinants of transposon Tn2555].
作者信息
Doroshenko V G, Danilevich V N, Livshits V A
出版信息
Mol Gen Mikrobiol Virusol. 1988 Nov(11):29-35.
The Sac- -derivatives of the plasmid pBRS5.2 containing the sucrose utilization transposon Tn2555 were obtained by using the insertional mutagenesis. The sac mutations were mapped within the transposons DNA fragment of about 3kb. The existence of two linkage groups of the sac mutations was shown by complementation analysis. Among the gene products of Tn2555 the polypeptides of about 58, 36, 27, 14 and 12 kd were found. The data on relation of the sac genes of Tn2555 and the ones of the known plasmid pUR400 of Salmonella typhimurium are discussed.
通过插入诱变获得了含有蔗糖利用转座子Tn2555的质粒pBRS5.2的Sac衍生物。Sac突变定位在约3kb的转座子DNA片段内。互补分析表明存在两个Sac突变连锁群。在Tn2555的基因产物中发现了约58、36、27、14和12kd的多肽。讨论了Tn2555的Sac基因与鼠伤寒沙门氏菌已知质粒pUR400的Sac基因之间的关系数据。
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