Doroshenko V G, Livshits V A
Mol Gen Mikrobiol Virusol. 1987 Jun(6):23-8.
Tn2555, a new transposon coding for genes of sucrose utilization was studied. Tn2555 was shown to integrate into the plasmids RP4 and R6K, phage P1CmClr100 and Escherichia coli K12 chromosome. Tn2555 frequency of transposition to RP4 and R6K DNA is (2-5) X 10(-7) in Rec+-strain, (3-6) X 10(-8) in Rec--strain. Tn2555 integration site in phage P1CmClr100 Sac+-derivative studied has been localised within the C-segment of P1 DNA. In three independent cases of Tn2555 transposition to the chromosome the transposon was found to be integrated in the region between 29 and 32 min of Escherichia coli K12 linkage map. The restriction endonuclease analysis of seven independent isolates of RP4::Tn2555 has shown the grouping of Tn2555 integration sites in the Tn1 region of RP4. Frequent rearrangements occurring within Tn2555 have been revealed by the analysis. However, an invertible DNA segment of about 6-7 kb was preserved in all transposon structures.
对编码蔗糖利用基因的新型转座子Tn2555进行了研究。结果表明，Tn2555可整合到质粒RP4和R6K、噬菌体P1CmClr100以及大肠杆菌K12染色体中。在Rec⁺菌株中，Tn2555转座到RP4和R6K DNA的频率为(2 - 5)×10⁻⁷，在Rec⁻菌株中为(3 - 6)×10⁻⁸。所研究的噬菌体P1CmClr100 Sac⁺衍生物中Tn2555的整合位点已定位在P1 DNA的C片段内。在Tn2555转座到染色体的三个独立案例中，发现转座子整合在大肠杆菌K12连锁图谱29至32分钟之间的区域。对七个独立的RP4::Tn2555分离株进行的限制性内切酶分析表明，Tn2555的整合位点在RP4的Tn1区域成组。分析揭示了Tn2555内部频繁发生的重排。然而，在所有转座子结构中都保留了一个约6 - 7 kb的可逆DNA片段。
