Azimzadeh Kaveh, Nasrollahi Nargesabad Reza, Vousooghi Nasim
Young Researcher and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran.
International Associate Member of Microbiology Society, London, UK.
Microb Pathog. 2017 Aug;109:67-70. doi: 10.1016/j.micpath.2017.05.031. Epub 2017 May 19.
Brucellosis is known as one of important zoonosis. Studying the histological and biochemical effects of the disease could help to increase our knowledge about it. The aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of Brucella (Brucella melitensis and Brucella abortus) and two vaccines (Rev-1, RB-51) in the rat. Forty male rats were divided into five groups (n = 8 in each group). Two groups received suspensions of Brucella abortus and Brucella melitensis and two other groups were injected intraperitoneally with two mentioned vaccines and the last group received only distilled water. The results showed a significant increase in sphingosine 1-phosphate, Malondialdehyde, hepcidin, homocysteine, cardiac troponin I and copper levels and a considerable decrease in the levels of iron and zinc (P ≤ 0.01) in infected groups compared to the control animals. In vaccinated groups, hepcidin was increased but other parameters were not changed in comparison to the control group. It can be concluded that increase of homocysteine and cardiac troponin I in brucellosis could be a warning for cardiac adverse effects. Besides, increase of sphingosine 1-phosphate probably indicates its stimulant and modulatory effects in anti- Brucellosis biochemical pathways of the host.
布鲁氏菌病是重要的人畜共患病之一。研究该疾病的组织学和生化影响有助于增加我们对它的了解。本研究的目的是评估大鼠腹腔注射两种布鲁氏菌（羊种布鲁氏菌和牛种布鲁氏菌）及两种疫苗（Rev-1、RB-51）后血浆参数的变化。40只雄性大鼠分为五组（每组n = 8）。两组接受牛种布鲁氏菌和羊种布鲁氏菌悬液，另外两组腹腔注射上述两种疫苗，最后一组仅接受蒸馏水。结果显示，与对照动物相比，感染组中鞘氨醇1-磷酸、丙二醛、铁调素、同型半胱氨酸、心肌肌钙蛋白I和铜水平显著升高，铁和锌水平显著降低（P≤0.01）。在接种疫苗组中，铁调素升高，但与对照组相比其他参数未发生变化。可以得出结论，布鲁氏菌病中同型半胱氨酸和心肌肌钙蛋白I的升高可能是心脏不良反应的警示信号。此外，鞘氨醇1-磷酸的升高可能表明其在宿主抗布鲁氏菌病生化途径中的刺激和调节作用。
