减毒活布鲁氏菌疫苗株中P39基因缺失对小鼠残余毒力和保护活性的影响。
Effect of P39 gene deletion in live Brucella vaccine strains on residual virulence and protective activity in mice.
作者信息
Tibor A, Jacques I, Guilloteau L, Verger J M, Grayon M, Wansard V, Letesson J J
机构信息
Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium.
出版信息
Infect Immun. 1998 Nov;66(11):5561-4. doi: 10.1128/IAI.66.11.5561-5564.1998.
Abstract
The 39-kilodalton protein (P39) has previously been shown to be an immunodominant protein in Brucella infections. P39 gene deletion mutants of vaccine strains Brucella abortus S19 and Brucella melitensis Rev.1 were constructed by gene replacement. This deletion did not significantly modify the residual virulence of both vaccine strains in CD-1 mice. CD-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. Mutant vaccine strains devoid of P39 could provide a means for differentiating vaccinated from infected animals.
摘要
39千道尔顿蛋白（P39）此前已被证明是布鲁氏菌感染中的一种免疫显性蛋白。通过基因替换构建了疫苗株流产布鲁氏菌S19和马尔他布鲁氏菌Rev.1的P39基因缺失突变体。这种缺失并未显著改变两种疫苗株在CD-1小鼠中的残余毒力。用亲本或突变株接种的CD-1小鼠对强毒攻击具有抵抗力。缺乏P39的突变疫苗株可为区分接种动物和感染动物提供一种方法。
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