Haydari Mohammad Reza, Panjeshahin Mohammad Reza, Mashghoolozekr Elaheh, Nekooeian Ali Akbar
Cardiovascular Pharmacology Research Lab, Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Med Sci. 2017 May;42(3):266-274.
Hawthorn species decreases blood pressure and relaxes precontracted vessels. This study aimed at examining the antihypertensive effect and related mechanisms of hydroalcoholic extract of fruit in rats with renovascular hypertension.
Six groups of male Sprague-Dawley rats, each containing 6 to 8 rats, were studied. The groups comprised of one sham group and 5 renal artery-clipped groups. The sham group received vehicle (distilled water 0.5 ml/day) and the renal artery-clipped groups received vehicle or the extract at 5, 10, 20 or 30 mg/kg/day. Oral vehicle or extract was administered daily for 4 weeks following sham-operation or induction of hypertension. Systolic blood pressure and heart rate were measured weekly. Isolated aorta study was performed by last week and serum superoxide dismutase and glutathione reductase were measured. The findings were analyzed using one-way analysis of variance and Duncan's multiple range tests at P≤0.05 using SigmaStat software.
The data obtained after 4 weeks of treatment showed that the renal artery-clipped group receiving vehicle had significantly higher systolic blood pressure (P=0.002) and phenylephrine maximal response (P=0.01); and lower acetylcholine maximal response (P=0.01), serum superoxide dismutase (P=0.006) and serum glutathione reductase (P=0.006) than those of the sham group. The renal artery-clipped group receiving extract had significantly lower systolic blood pressure (P=0.03) and phenylephrine maximal response (P=0.01); and significantly higher acetylcholine maximal response (P=0.01), serum superoxide dismutase (P=0.015), and serum glutathione reductase (P=0.015) than those of the renal artery-clipped group receiving vehicle.
Our findings show that the hydroalcoholic extract of fruit has antihypertensive effects, which may be partly due to antioxidant and nitric oxide releasing effects.
山楂属植物可降低血压并舒张预收缩的血管。本研究旨在探讨山楂果实水醇提取物对肾血管性高血压大鼠的降压作用及相关机制。
研究选用6组雄性Sprague-Dawley大鼠，每组6至8只。分组包括1个假手术组和5个肾动脉夹闭组。假手术组给予赋形剂（蒸馏水0.5毫升/天），肾动脉夹闭组给予赋形剂或提取物，剂量分别为5、10、20或30毫克/千克/天。在假手术或诱导高血压后，每天口服给予赋形剂或提取物，持续4周。每周测量收缩压和心率。在最后一周进行离体主动脉研究，并测量血清超氧化物歧化酶和谷胱甘肽还原酶。使用SigmaStat软件，采用单因素方差分析和Duncan多重极差检验对结果进行分析，P≤0.05。
治疗4周后获得的数据显示，接受赋形剂的肾动脉夹闭组的收缩压（P = 0.002）和去氧肾上腺素最大反应（P = 0.01）显著高于假手术组；乙酰胆碱最大反应（P = 0.01）、血清超氧化物歧化酶（P = 0.006）和血清谷胱甘肽还原酶（P = 0.006）低于假手术组。接受提取物的肾动脉夹闭组的收缩压（P = 0.03）和去氧肾上腺素最大反应（P = 0.01）显著低于接受赋形剂的肾动脉夹闭组；乙酰胆碱最大反应（P = 0.01）、血清超氧化物歧化酶（P = 0.015）和血清谷胱甘肽还原酶（P = 0.015）显著高于接受赋形剂的肾动脉夹闭组。
我们的研究结果表明，山楂果实水醇提取物具有降压作用，这可能部分归因于抗氧化和释放一氧化氮的作用。
