Hernández-Pérez Abigail, Bah Moustapha, Ibarra-Alvarado César, Rivero-Cruz José Fausto, Rojas-Molina Alejandra, Rojas-Molina Juana Isela, Cabrera-Luna José Alejandro
Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico.
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Circuito exterior, Coyoacán 04510, Mexico D.F., Mexico.
Molecules. 2014 Dec 15;19(12):20962-74. doi: 10.3390/molecules191220962.
This study focused on the assessment of the vasorelaxant activity of the organic and aqueous extracts obtained from leaves and fruits of a Mexican hawthorn (Crataegus gracilior) on isolated rat aorta, and on the purification and identification of some of their secondary metabolites by the use of chromatographic and spectroscopic techniques. The results obtained showed that the methanol extract has a significantly more potent and effective vasorelaxant effect than the other tested extracts, with an EC50 = 8.69 ± 4.34 µg/mL and an Emax = 94.6% ± 11.30%, values that are close to that of acetylcholine, the positive control. From the same extract, two major triterpenes were isolated and identified as ursolic and corosolic acids by comparison of their experimental NMR spectroscopic data with those reported in the literature. Chlorogenic acid, rutin, quercetin, kaempferol and (+)-catechin were also identified using HPLC coupled with PDAD. All these compounds have already been proven to possess on their own antihypertensive effect and other benefits on cardiovascular diseases and they can support, at least in part, the traditional use of this plant species.
本研究聚焦于评估从墨西哥山楂（Crataegus gracilior）的叶子和果实中获得的有机提取物和水提取物对离体大鼠主动脉的血管舒张活性，并利用色谱和光谱技术对其一些次生代谢产物进行纯化和鉴定。所得结果表明，甲醇提取物比其他受试提取物具有显著更强且更有效的血管舒张作用，其半数有效浓度（EC50）= 8.69 ± 4.34 µg/mL，最大效应（Emax）= 94.6% ± 11.30%，这些值与阳性对照乙酰胆碱的值相近。从同一提取物中分离出两种主要的三萜类化合物，并通过将其实验核磁共振光谱数据与文献报道的数据进行比较，鉴定为熊果酸和科罗索酸。还使用高效液相色谱联用光电二极管阵列检测器（HPLC - PDAD）鉴定了绿原酸、芦丁、槲皮素、山奈酚和（+）-儿茶素。所有这些化合物已被证明自身具有降压作用以及对心血管疾病的其他益处，并且它们至少可以部分支持该植物物种的传统用途。
