Samutela Mulemba Tillika, Kalonda Annie, Mwansa James, Lukwesa-Musyani Chileshe, Mwaba John, Mumbula Enoch Mulowa, Mwenya Darlington, Simulundu Edgar, Kwenda Geoffrey
Department of Biomedical Sciences, School of Medicine, University of Zambia,P.O. Box 50110, Lusaka, Zambia.
Department of Pathology and Microbiology, University Teaching Hospital, P/Bag RW X1 Lusaka, Zambia.
Pan Afr Med J. 2017 Feb 28;26:108. doi: 10.11604/pamj.2017.26.108.10982. eCollection 2017.
Methicillin-resistant (MRSA) is globally recognized as an important public health problem. Whereas comprehensive molecular typing data of MRSA strains is available, particularly in Europe, North America and Australia, similar information is very limited in sub-Saharan Africa including Zambia.
In this study, thirty two clinical isolates of , collected at a large referral hospital in Lusaka, Zambia between June 2009 and December 2012 were analysed by Staphylococcal cassette chromosome protein A gene typing and detection of the Panton-Valentine Leukocidin genes .
Three SCC types were identified namely SCC type IV (65.6%), SCCmec type III (21.9%), SCC type I (3.1%). Nine point four percent (9.4%) of the isolates were untypable. Five types, which included a novel type, were detected and the most prevalent spa type was t064 (40.6%). Other types included spa types t2104 (31.3%), t355 (3.1%) and t1257 (21.9%). The genes were detected in 3 out of 32 isolates.
These molecular typing data indicated that the MRSA strains collected in Lusaka were diverse. Although the source of these MRSA was not established, these results stress the need for assessing infection prevention and control procedures at this health-care facility in order to curtail possible nosocomial infections. Furthermore, country-wide surveillance of MRSA in both the community and health-care facilities is recommended for infection prevention and control. To our knowledge, this represents the first study to characterise MRSA using molecular tools in Zambia.
耐甲氧西林金黄色葡萄球菌（MRSA）在全球范围内被公认为是一个重要的公共卫生问题。尽管有MRSA菌株的全面分子分型数据，尤其是在欧洲、北美和澳大利亚，但在包括赞比亚在内的撒哈拉以南非洲，类似信息非常有限。
在本研究中，对2009年6月至2012年12月期间在赞比亚卢萨卡一家大型转诊医院收集的32株临床分离株进行葡萄球菌盒式染色体蛋白A基因分型和杀白细胞素基因检测。
鉴定出三种SCC类型，即IV型（65.6%）、III型（21.9%）、I型（3.1%）。9.4%的分离株无法分型。检测到五种spa类型，其中包括一种新型，最常见的spa类型是t064（40.6%）。其他spa类型包括t2104（31.3%）、t355（3.1%）和t1257（21.9%）。在32株分离株中有3株检测到lukS-PV基因。
这些分子分型数据表明，在卢萨卡收集的MRSA菌株具有多样性。尽管这些MRSA的来源尚未确定，但这些结果强调需要评估该医疗机构的感染预防和控制程序，以减少可能的医院感染。此外，建议在全国范围内对社区和医疗机构中的MRSA进行监测，以预防和控制感染。据我们所知，这是赞比亚首次使用分子工具对MRSA进行特征分析的研究。
