Li Jinquan, Xu Li, Bao Zhen, Xu Peng, Chang Hao, Wu Jingjing, Bei Yuanqi, Xia Liuwan, Wu Peizhang, Cui Gang
Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Targets, Nantong University, Nantong, Jiangsu 226001, P.R. China.
Oncol Rep. 2017 Jul;38(1):183-192. doi: 10.3892/or.2017.5647. Epub 2017 May 17.
Piwi-like RNA-mediated gene silencing 2 (PIWIL2), has been reported as an oncogene tightly associated with the genesis and progression of various malignancies. Nevertheless, the function of the PIWIL2 protein in human gliomas has not yet been clarified. In this study, we sought to investigate the clinical significance of PIWIL2 expression and reveal its function in the pathological process of gliomas. Through western blot and immunohistochemical analyses we found that PIWIL2 was overexpressed in glioma tissues. Moreover, the expression level of PIWIL2 was also significantly correlated with the WHO grades of human gliomas and Ki-67 expression. Kaplan‑Meier curves indicated that PIWIL2 was a prognostic factor for the survival of glioma patients and a high expression of PIWIL2 was correlated with a poor prognosis. In vitro, knockdown of PIWIL2 in glioma cells was shown to induce cell cycle arrest and increase apoptosis. Furthermore, silencing of PIWIL2 expression also obviously suppressed the migration of glioma cells. All the results demonstrated that PIWIL2 plays a significant role in the pathogenesis of human gliomas and may be used as a potential diagnostic marker and a therapeutic target of glioma in the future.
Piwi样RNA介导的基因沉默2（PIWIL2）已被报道为一种与多种恶性肿瘤的发生和进展密切相关的癌基因。然而，PIWIL2蛋白在人类胶质瘤中的功能尚未阐明。在本研究中，我们试图探讨PIWIL2表达的临床意义，并揭示其在胶质瘤病理过程中的作用。通过蛋白质印迹和免疫组织化学分析，我们发现PIWIL2在胶质瘤组织中过表达。此外，PIWIL2的表达水平也与人类胶质瘤的WHO分级和Ki-67表达显著相关。Kaplan-Meier曲线表明，PIWIL2是胶质瘤患者生存的一个预后因素，PIWIL2的高表达与不良预后相关。在体外，胶质瘤细胞中PIWIL2的敲低显示可诱导细胞周期停滞并增加细胞凋亡。此外，PIWIL2表达的沉默也明显抑制了胶质瘤细胞的迁移。所有结果表明，PIWIL2在人类胶质瘤的发病机制中起重要作用，未来可能用作胶质瘤的潜在诊断标志物和治疗靶点。
