Höchli M, Höchli L, Hackenbrock C R
Eur J Cell Biol. 1985 Jul;38(1):1-5.
Distinct fluorophores have been conjugated to antibodies for cytochrome bc1 complex and cytochrome oxidase, two integral electron transferring proteins in the mitochondrial inner membrane. Addition of these fluorescent antibodies to preparations of mitochondrial inner membranes followed by appropriate secondary antibodies causes distinct and independent aggregation of the two cytochrome proteins. These results reveal that both cytochrome bc1 complex and cytochrome oxidase diffuse laterally in the membrane plane independent of one another consistent with the random collision model for electron transport in the mitochondrial inner membrane.
不同的荧光团已与细胞色素bc1复合体和细胞色素氧化酶的抗体偶联，这两种蛋白是线粒体内膜中两个完整的电子传递蛋白。将这些荧光抗体添加到线粒体内膜制剂中，随后加入合适的二抗，会导致这两种细胞色素蛋白发生明显且独立的聚集。这些结果表明，细胞色素bc1复合体和细胞色素氧化酶在膜平面内彼此独立地横向扩散，这与线粒体内膜中电子传递的随机碰撞模型一致。
