冷冻保存的间充质干细胞促进椎间盘再生。
Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc.
作者信息
Volkova Nataliia, Yukhta Mariia, Goltsev Anatoliy
机构信息
Laboratory of Biotechnology and Applied Nanotechnology, Department of Cryobiology of Reproductive System, Institute for Problems of Cryobiology and Сryomedicine of the National Academy of Sciences of Ukraine, Kharkov 61015, Ukraine.
出版信息
Biomedicines. 2015 Aug 7;3(3):237-247. doi: 10.3390/biomedicines3030237.
BACKGROUND
Degenerative diseases are a medical, social, and economic problem worldwide. The most significant factors predisposing the development of degenerative changes in intervertebral discs are a low density and poor biosynthetic potential of the cells. Therefore, stem cell therapy in this case should show high clinical efficiency.
METHODS
The research aim was to evaluate the regenerative potential of cryopreserved mesenchymal stem cells (MSCs) upon degenerative changes in intervertebral discs. Rats with simulated degenerative damage of the intervertebral disc Co6-Co7 were administrated with 0.5 × 10⁶ of either native or cryopreserved cells on a collagen sponge to the defect area. The results of experiments were histomorphometrically evaluated on the 30th, 60th, and 90th days after treatment.
RESULTS
The restoration of tears, clefts, and collagen fiber fragmentations was noted on the 60th and 90th day after administration of native and cryopreserved MSCs respectively. An increase in fibrochondrocyte density got ahead of the annulus fibrosus height recovery. In the control group without treatment the regeneration was hardly observed.
CONCLUSION
The use of MSCs promotes the restoration of the degenerated intervertebral disc. Cryopreserved MSCs have a "lag" therapeutic effect at the early stages, but show similar results to the native analogue on the 90th day after administration.
背景
退行性疾病是一个全球性的医学、社会和经济问题。导致椎间盘发生退行性改变的最重要因素是细胞密度低和生物合成潜力差。因此，在这种情况下干细胞治疗应具有较高的临床疗效。
方法
研究目的是评估冷冻保存的间充质干细胞（MSCs）对椎间盘退行性改变的再生潜力。将模拟Co6-Co7椎间盘退行性损伤的大鼠在缺损区域的胶原海绵上分别植入0.5×10⁶个天然或冷冻保存的细胞。在治疗后第30、60和90天对实验结果进行组织形态计量学评估。
结果
分别在给予天然和冷冻保存的MSCs后第60天和第90天观察到撕裂、裂隙和胶原纤维断裂的修复。纤维软骨细胞密度的增加先于纤维环高度的恢复。在未治疗的对照组中几乎未观察到再生。
结论
使用MSCs可促进退变椎间盘的修复。冷冻保存的MSCs在早期具有“滞后”的治疗效果，但在给药后第90天显示出与天然类似物相似的结果。
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