Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
Department of Neurosciences, San Camillo-Forlanini Hospital, Rome, Italy.
Mult Scler. 2018 Jul;24(8):1039-1045. doi: 10.1177/1352458517711774. Epub 2017 May 24.
Brain atrophy is a known marker of irreversible tissue damage in multiple sclerosis (MS). Cerebrospinal fluid (CSF) osteopontin (OPN) and neurofilament light chain (NF-L) have been proposed as candidate surrogate markers of inflammatory and neurodegenerative processes in MS.
To evaluate the relationship between CSF NF-L and OPN levels and brain grey and white matter volumes in patients with clinically isolated syndrome (CIS) suggestive of MS.
A total of 41 CIS patients and 30 neurological controls (NCs) were included. CSF NF-L and OPN were measured by commercial ELISA. Measures of brain volume (normalized brain volume (NBV), normalized grey matter volume (NGV), peripheral grey matter volume (PGV), normalized white matter volume (WMV), and ventricular volume) were obtained by SIENAX. Corpus callosum index (CCI) was calculated. Brain volumes were categorized into 'high' and 'low' according to the median value.
CSF NF-L and OPN levels were higher in CIS patients in comparison with NCs. CIS patients with 'low' TGV, PGV, and TBV showed higher CSF NF-L levels than CIS patients with 'high' brain volumes. TGV and PGV correlated inversely with NF-L levels, whereas CCI was inversely related to OPN levels. CSF NF-L was the only independent predictor of TGV and PGV.
CSF NF-L tracks mainly grey matter damage in patients with CIS suggestive of MS.
脑萎缩是多发性硬化症（MS）中已知的不可逆组织损伤标志物。脑脊液（CSF）中的骨桥蛋白（OPN）和神经丝轻链（NF-L）已被提议作为 MS 中炎症和神经退行性过程的候选替代标志物。
评估临床孤立综合征（CIS）患者中 CSF NF-L 和 OPN 水平与脑灰质和白质体积之间的关系，这些患者提示 MS。
共纳入 41 名 CIS 患者和 30 名神经对照组（NCs）。通过商业 ELISA 测量 CSF NF-L 和 OPN。脑容量的测量指标（标准化脑容量（NBV）、标准化灰质容量（NGV）、外周灰质容量（PGV）、标准化白质容量（WMV）和脑室容量）通过 SIENAX 获得。计算胼胝体指数（CCI）。根据中位数将脑容量分为“高”和“低”。
与 NCs 相比，CIS 患者的 CSF NF-L 和 OPN 水平更高。与脑容量较高的 CIS 患者相比，脑容量较低的 CIS 患者的 CSF NF-L 水平更高。TGV 和 PGV 与 NF-L 水平呈负相关，而 CCI 与 OPN 水平呈负相关。CSF NF-L 是 TGV 和 PGV 的唯一独立预测因子。
CSF NF-L 主要追踪 CIS 患者的灰质损伤，这些患者提示 MS。
