MTA-PTE Clinical Neuroscience MR Research Group, Eötvös Loránd Research Network (ELKH), Ret str. 2, 7623, Pecs, Hungary.
Department of Neurology, Medical School, University of Pecs, Pecs, Hungary.
Sci Rep. 2021 Dec 8;11(1):23604. doi: 10.1038/s41598-021-03173-3.
Osteopontin (OPN) is a proinflammatory marker produced by systemic immune and central nervous system (CNS) resident cells. We examined, if the level of OPN in the cerebrospinal fluid (CSF) and blood is associated with late-time regional brain volumes and white matter (WM) lesion load in MS. Concentrations of OPN in blood and CSF were related to MRI findings 10.1 ± 2.0 years later in 46 patients with MS. OPN concentration was measured by ELISA, while regional brain volumes and lesion load was assessed by magnetic resonance imaging (MRI) using 3D MPRAGE sequence and automated MR volumetry. OPN measured in the CSF was associated with several regional brain volumes and WM lesion load measured 10.1 ± 2.0 years later. CSF OPN concentration correlated with long-term enlargement of lateral- and inferior lateral ventricles and the elevation of gross CSF volume, in conjunction with the reduction of several cortical/subcortical gray matter and WM volumes. Serum OPN showed no long-term association with regional brain volumes. OPN measured from the CSF but not from the serum was associated with lower regional brain volumes measured a decade later, indicating the primary role of inflammation within the CNS in developing long-term brain related alterations.
骨桥蛋白 (OPN) 是一种由全身免疫细胞和中枢神经系统 (CNS) 固有细胞产生的促炎标志物。我们研究了脑脊液 (CSF) 和血液中的 OPN 水平是否与 MS 患者的晚期区域性脑容量和白质 (WM) 病变负荷有关。在 46 例 MS 患者中，OPN 在血液和 CSF 中的浓度与 10.1±2.0 年后的 MRI 发现相关。OPN 浓度通过 ELISA 测量，而区域性脑容量和病变负荷通过磁共振成像 (MRI) 使用 3D MPRAGE 序列和自动 MR 容积测量来评估。CSF 中测量的 OPN 与 10.1±2.0 年后测量的几个区域性脑容量和 WM 病变负荷相关。CSF OPN 浓度与侧脑室和下外侧脑室的长期扩大以及总 CSF 体积的升高相关，同时伴有几个皮质/皮质下灰质和 WM 体积的减少。血清 OPN 与区域性脑容量无长期关联。CSF 中测量的 OPN 而不是血清中测量的 OPN 与 10 年后测量的区域性脑容量较低有关，表明 CNS 内的炎症在发展长期与大脑相关的改变方面起主要作用。
