[转录因子NF-kB、HIF-1、HIF-2、生长因子VEGF、VEGFR2和碳酸酐酶IX mRNA及蛋白水平在肾癌转移发生过程中的变化]
[Transcription factors NF-kB, HIF-1, HIF-2, growth factor VEGF, VEGFR2 and carboanhydrase IX mRNA and protein level in the development of kidney cancer metastasis].
作者信息
Spirina L V, Usynin Y A, Yurmazov Z A, Slonimskaya E M, Kolegova E S, Kondakova I V
机构信息
Tomsk Cancer Research Institute Tomsk, 634050 Russia.
Siberian State Medical University Tomsk, 634050 Russia.
出版信息
Mol Biol (Mosk). 2017 Mar-Apr;51(2):372-377. doi: 10.7868/S0026898417020197.
Here, we have investigated the participation of nuclear factors NF-kB, HIF-1 and HIF-2, VEGF, VEGFR2, and carboanhydrase IX in clear-cell renal cancer. We have determined the expression and protein level of transcription factors, VEGF, VEGFR2, and carboanhydrase IX in tumor and normal tissues of 30 patients with kidney cancer. The Real-Time PCR and ELISA were used in the study. The low levels of HIF-1 mRNA expression associated with high levels of HIF-1 protein were also associated with metastasis. The expression levels of VEGF, VEGFR2, and their protein levels are increased in primary tumors of patients with disseminated kidney cancer compared to nonmetastatic cancer. No correlation was revealed between the content of mRNA and encoded proteins in the kidney cancer tissues. The changes in the ratios of mRNA levels and the respective proteins (HIF-1α, HIF-2, NF-kB, VEGF, VEGFR2, and carboanhydrase IX) may contribute to kidney-cancer metastasis.
在此，我们研究了核因子NF-κB、低氧诱导因子-1（HIF-1）和低氧诱导因子-2（HIF-2）、血管内皮生长因子（VEGF）、血管内皮生长因子受体2（VEGFR2）以及碳酸酐酶IX在透明细胞肾癌细胞中的作用。我们测定了30例肾癌患者肿瘤组织和正常组织中转录因子、VEGF、VEGFR2以及碳酸酐酶IX的表达和蛋白水平。本研究采用了实时定量聚合酶链反应（Real-Time PCR）和酶联免疫吸附测定（ELISA）。低水平的HIF-1信使核糖核酸（mRNA）表达与高水平的HIF-1蛋白相关，且也与转移相关。与非转移性癌症相比，在播散性肾癌患者的原发性肿瘤中，VEGF、VEGFR2的表达水平及其蛋白水平均升高。在肾癌组织中，未发现mRNA含量与编码蛋白之间存在相关性。mRNA水平与相应蛋白（HIF-1α、HIF-2、NF-κB、VEGF、VEGFR2和碳酸酐酶IX）比例的变化可能促进肾癌转移。
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