Sun Wen-Wen, Dou Jin-Xia, Zhang Lin, Qiao Li-Kui, Shen Na, Zhao Qiang, Gao Wen-Yuan
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Institute of Fundamental Research, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China.
Oncotarget. 2017 Jul 11;8(28):46081-46089. doi: 10.18632/oncotarget.17687.
This study aims to compare the efficacy of different treatments for nude mice transplanted with HT-29 colon carcinoma cell line. BalB/C nude mice were transplanted with HT-29 colon carcinoma cell line and randomly divided into four groups, with 5 mice in each group: blank control group, DC-CIK group, Huaier Granule group, and Huaier Granule group combined with DC-CIK group (combined treatment group). For DC-CIK group and combined treatment group, 1×106 DC-CIK cells were injected via the tail vein 4 days after transplantation. The injection was performed twice weekly for a total of 2 weeks. For Huaier Granule group and combined treatment group, Huaier Granule was administered at the dose of 20 g/60 g, by dissolving 20 g of Huaier granules in 600 ml of pure water. Intragastric administration of 0.2 ml of granules was performed once daily for 3 weeks. For the blank control group, equal volume of normal saline was given. Tumor size and body weight of nude mice were measured every 2 days during the 3-week treatment. The mice were sacrificed at the end of treatment to harvest tumors. Key genes of the signaling pathway were detected by RT-PCR. At the end of treatment, mice in combined treatment group, DC-CIK group and Huaier Granule group remained stable emotionally with normal mobility and water and food intake. However, in the blank control group, the mobility was restricted starting from the third week and the mice were on the verge of dying. The expression of PI3KR1, Akt, Wnt1, CTTNB1, Notch1, Notch2 and Notch3 genes were all downregulated significantly in the combined treatment group compared with DC-CIK group and Huaier Granule group (P<0.05). Therefore, the combined treatment of Huaier Granule combined with DC-CIK achieved the best effect in nude mice transplanted with HT-29 colon carcinoma cell line.
本研究旨在比较不同治疗方法对移植了HT - 29结肠癌细胞系的裸鼠的疗效。将BalB/C裸鼠移植HT - 29结肠癌细胞系后随机分为四组，每组5只：空白对照组、DC - CIK组、槐耳颗粒组以及槐耳颗粒联合DC - CIK组（联合治疗组）。对于DC - CIK组和联合治疗组，移植后4天经尾静脉注射1×10⁶个DC - CIK细胞。每周注射两次，共注射2周。对于槐耳颗粒组和联合治疗组，将20 g槐耳颗粒溶解于600 ml纯水中，以20 g/60 g的剂量给予槐耳颗粒。每日灌胃0.2 ml颗粒，共3周。对于空白对照组，给予等量的生理盐水。在3周的治疗期间，每2天测量裸鼠的肿瘤大小和体重。治疗结束时处死小鼠以收获肿瘤。通过RT - PCR检测信号通路的关键基因。治疗结束时，联合治疗组、DC - CIK组和槐耳颗粒组的小鼠情绪稳定，活动、饮水和进食正常。然而，空白对照组从第三周开始活动受限，小鼠濒临死亡。与DC - CIK组和槐耳颗粒组相比，联合治疗组中PI3KR1、Akt、Wnt1、CTTNB1、Notch1、Notch2和Notch3基因的表达均显著下调（P<0.05）。因此，槐耳颗粒联合DC - CIK的联合治疗在移植了HT - 29结肠癌细胞系的裸鼠中效果最佳。
