Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China; Research and Development Department, Guangdong Qizhi Biotechnology Co., Ltd., Guangzhou, PR China.
Bioresour Technol. 2017 Sep;239:402-411. doi: 10.1016/j.biortech.2017.04.053. Epub 2017 Apr 18.
Sodium hydroxide pretreatment of sugarcane bagasse under vacuum conditions was established and evaluated in this study. Compared to pretreatment under conventional moderate pressure conditions, only half of the total phenolic compounds and less than half of the formic acid were produced under vacuum conditions, while the yield of total fermentable sugar was significantly increased by 31.38%. The pretreatment parameters: NaOH concentration, pretreatment time, and pretreatment temperature, were optimized using response surface methodology based on the response values of the total fermentable sugar yield (TFSY) and the total fermentable sugar concentration (TFSC), respectively. Under the optimal conditions, the TFSY of 0.5146g/g and the TFSC of 17.37g/L were achieved, respectively. By adjusting the ratio of cellulases to xylanase, the TFSY reached a maximum of 0.5213g/g when the ratio was 1:1, while the maximum TFSC of 17.71g/L was achieved when the ratio was 1:4.
本研究建立并评估了在真空条件下对甘蔗渣进行氢氧化钠预处理的方法。与常规中压条件下的预处理相比，真空条件下仅产生了总酚类化合物的一半和甲酸的不到一半，而总可发酵糖的得率却显著提高了 31.38%。通过响应面法，根据总可发酵糖得率（TFSY）和总可发酵糖浓度（TFSC）的响应值，分别对预处理参数：NaOH 浓度、预处理时间和预处理温度进行了优化。在最佳条件下，分别获得了 0.5146g/g 的 TFSY 和 17.37g/L 的 TFSC。通过调整纤维素酶与木聚糖酶的比例，当比例为 1:1 时，TFSY 达到最大值 0.5213g/g，而当比例为 1:4 时，TFSC 达到最大值 17.71g/L。
