Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Bioresour Technol. 2018 Feb;249:226-233. doi: 10.1016/j.biortech.2017.09.137. Epub 2017 Sep 22.
Sodium methoxide (CHONa) with glycerol pretreatment (CWGP) was performed to improve the enzymatic digestibility of sugarcane bagasse (SCB). Response surface methodology was utilized to optimize the CWGP parameters for pretreating SCB from the perspective of total fermentable sugar yield (TFSY) and total fermentable sugar concentration (TFSC). Under the optimal CWGP conditions, 0.5666g/g of TFSY (0.82% CHONa, 1.11h, 150°C) and 17.75g/L of TFSC (0.87% CHONa, 1.38h, 149.27°C) were achieved, corresponding to delignification of 79.05% and 79.34%, respectively. Compared the pretreatment using glycerol or CHONa alone, the CWGP has significant synergies to enhance the enzymatic efficiency of SCB. The physical and chemical characteristics of untreated and pretreated SCBs were analyzed using FT-IR, XRD, and SEM, and the results suggest that CWGP significantly increased the susceptibility of the substrates to enzymatic digestibility. Ultimately, CWGP might be a prospective candidate for the pretreatment process of enzyme-based lignocellulosic biorefineries.
采用甲醇钠（CHONa）与甘油预处理（CWGP）来提高甘蔗渣（SCB）的酶解可消化性。从总可发酵糖产率（TFSY）和总可发酵糖浓度（TFSC）的角度，利用响应面法对 CWGP 预处理 SCB 的参数进行优化。在最优的 CWGP 条件下，实现了 0.5666g/g 的 TFSY（0.82% CHONa，1.11h，150°C）和 17.75g/L 的 TFSC（0.87% CHONa，1.38h，149.27°C），对应的脱木质素率分别为 79.05%和 79.34%。与单独使用甘油或 CHONa 的预处理相比，CWGP 具有显著的协同作用，可提高 SCB 的酶解效率。利用傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对未处理和预处理的 SCB 的物理化学特性进行了分析，结果表明 CWGP 显著提高了底物对酶解的敏感性。总之，CWGP 可能是基于酶的木质纤维素生物炼制预处理过程的有前途的候选物。
