Brasch M A, Meyer R J
Department of Microbiology, University of Texas, Austin 78712-1095.
Mol Gen Genet. 1988 Dec;215(1):139-45. doi: 10.1007/BF00331315.
Replication of plasmid R1162 DNA does not require the product of the dnaA gene. An integrated copy of the plasmid can suppress the temperature-sensitive dnaA46 allele when (1) additional plasmid copies are present in the cytoplasm and (2) an inactive replication origin, generated by deletion, is also present in the chromosome. We propose that the inactive origin sets the rate of initiation of chromosome replication at a level compatible with cell viability, possibly by providing additional binding sites for an R1162-encoded protein that is rate-limiting for plasmid replication.
质粒R1162 DNA的复制不需要dnaA基因的产物。当（1）细胞质中存在额外的质粒拷贝，且（2）染色体中也存在由缺失产生的无活性复制起点时，该质粒的整合拷贝可以抑制温度敏感型dnaA46等位基因。我们提出，无活性起点将染色体复制的起始速率设定在与细胞活力相适应的水平，可能是通过为一种对质粒复制起限速作用的R1162编码蛋白提供额外的结合位点来实现的。
